Czech J. Food Sci., 2009, 27(5):320-329 | DOI: 10.17221/48/2008-CJFS

Influence of glycation and pepsin hydrolysis on immunoreactivity of albumin/globulin fraction of herbicide resistant wheat line

András Nagy1, 2, Katarzyna Marciniak-Darmochwa³2, Stanis³aw Krawczuk3, Dagmara Mierzejewska2, Henryk Kostyra2, Éva Gelencsér1
1 Department of Biology, Central Food Research Institute, Budapest, Hungary
2 Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland
3 Faculty of Biology, University of Warmia and Mazury, Olsztyn, Poland

The aim of this study was to investigate the influence of non-enzymatic glycosylation on the immunogenic properties of soluble wheat proteins. Albumin/globulin fractions of herbicide resistant wheat line were non-enzymatically glycosylated using glucose for seven days at 37°C. The changes in their structures and immunoreactivity were then determined. The protein fractions were also hydrolysed with pepsin to determine the resistance to digestion. Albumin/globulin fractions before and after non-enzymatic glycosylation were analysed using o-phthaldialdehyde method and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The immunoreactivity of the protein fractions was determined using enzyme-linked immunosorbent assay methods, as well as affinity chromatography. The soluble wheat proteins showed smaller amounts of available α-amino groups after non-enzymatic glycosylation, and were stronger immunogens after glycation, but their antigenicity was not been affected significantly. However, pepsin hydrolysis of wheat proteins decreased their immunoreactivity.

Keywords: non-enzymatic glycosylation; glycation; pepsin hydrolysis; immunoreactivity

Published: October 31, 2009  Show citation

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Nagy A, Marciniak-Darmochwa³ K, Krawczuk S, Mierzejewska D, Kostyra H, Gelencsér É. Influence of glycation and pepsin hydrolysis on immunoreactivity of albumin/globulin fraction of herbicide resistant wheat line. Czech J. Food Sci. 2009;27(5):320-329. doi: 10.17221/48/2008-CJFS.
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