Czech J. Food Sci., 2009, 27(4):284-292 | DOI: 10.17221/84/2009-CJFS

The occurrence of enterotoxigenic isolates of B. cereus in foodstuffs

Josef Brychta1, Jiří Smola2, Petr Pipek3, Jaroslav Ondráček2, Vladimír Bednář2, Alois Čížek3, Tomáš Brychta3
1 State Veterinary Institute, Jihlava, Czech Republic
2 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic
3 Department of Food Preservation and Meat Technology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic

Enterotoxigenic Bacillus cereus was detected in a variety of meat stuffs (36), ready-to-cook products (5), and swabs (7). The bacterial colonies isolated from PEMBA agar were identified as B. cereus. The 85 isolates were examined for the enterotoxin production using both TECRA-VIA and BCET-RPLA kits (ELISA - 47, RPLA - 38). Thirty two isolates (66%) were positive for enterotoxin using the ELISA test while only 15 isolates (39%) gave positive results in the RPLA test system. In total, 178 (91.8%) and 164 (84%) of the strains isolated in our laboratory (from various foods) were enterotoxigenic as determined using TECRA-VIA and BCET-RPLA, respectively. Parallel enterotoxin positive results obtained using both tests were demonstrated in only 9 isolates from 19 assessed (47.4%). Coincidental negative results from both kits were established for 3 isolates (15.8%) only. The isolates of B.cereus from meat were resistant to cephalothin (57%), clindamycin (14%), oxytetracycline (14%), and erythromycin (7%). The isolates from swabs were resistant to cephalothin (83%), erythromycin (16%), clindamycin (16%) and enrofloxacin (16%).

Keywords: Bacillus cereus; enterotoxin; bacterial resistance; foodstuffs; ELISA; RPLA

Published: August 31, 2009  Show citation

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Brychta J, Smola J, Pipek P, Ondráček J, Bednář V, Čížek A, Brychta T. The occurrence of enterotoxigenic isolates of B. cereus in foodstuffs. Czech J. Food Sci. 2009;27(4):284-292. doi: 10.17221/84/2009-CJFS.
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    References

    1. Ahmed R., Sankar-Mistry P., Jackson S., Ackermann H.W. Kasatiya S.S. (1995): Bacillus cereus phage typing as an epidemiological tool in outbreaks of food poisoning. Journal of Clinical Microbiology. 33: 636-640. Go to original source... Go to PubMed...
    2. Anonymous (1995): AOAC. Differentiation of members of Bacillus cereus group: Microbiological method. Sec. 17.8.02, Method 983.26. In: Cunniff P.A. (ed.): Official Methods of Analysis of AOAC International. 16th Ed. AOAC International, Gaithersburg: 54-55.
    3. Anonymous (2003a): Bacillus cereus RPLA Toxin Detection Kit (BCET-RPLA TD950).
    4. Anonymous (2003b): Bacillus cereus. OXOID Manual, No. 4: 21-38.
    5. Batt C.A. (1998): A risk assessment approach for foodborne Bacillus cereus and its toxins. Journal of Applied Microbiology (Symposium Supplement), 84: 51-61. Go to original source... Go to PubMed...
    6. Beecher D.J., Wong A.C.L. (1997): Tripartite hemolysin BL from Bacillus cereus. Journal of Biological Chemistry, 272: 233-239. Go to original source... Go to PubMed...
    7. Beecher D.J., Pulido J.S., Barney N.P., Wong A.C.L. (1995): Extracellular virulence factors in Bacillus cereus endophthalmitis: Methods and implication of involvement of hemolysin BL. Infection and Immunity, 63: 632-639. Go to original source... Go to PubMed...
    8. Braun P., Fehlhaber K., Klug C., Kopp K. (1999): Investigations into the activity of enzymes produced by spoilage-causing bacteria: a possible basis for improved shelf-life estimation. Food Microbiology, 16: 531-540. Go to original source...
    9. Champagne C.P., Laing R.R., Roy D., Mafu A A., Griffiths M. (1994): Psychrotrops in dairy products: their effects and their control. Critical Reviews in Food Science and Nutrition, 34: 1-30. Go to original source... Go to PubMed...
    10. Christiansson A., Naidu A.S., Nilsson I., Wadstrom T., Pettersson H.E. (1989): Toxin production by Bacillus cereus dairy isolates in milk at low temperatures. Applied and Environmental Microbiology, 55: 2595-2600. Go to original source... Go to PubMed...
    11. Ehling-Schulz M., Svensson B., Guinebretiere M.-H., Lindbäck T., Andersson M., Schulz A., Fricker M., Christiansson A., Granum P.E., Märtlbauer E., Nguyen-The Ch., Salkinoja-Salonen M., Scherer S. (2005): Emetic toxin formation of Bacillus cereus is restricted to a single evolutionary lineage of closely related strains. Microbiology. 151: 183-197. Go to original source... Go to PubMed...
    12. Flint S., Bremer P.J., Brooks J.D. (1997): Biofilms in dairy manufacturing plant - description, current concerns and methods of control. Biofouling, 11: 81-97. Go to original source...
    13. Flint S., Palmer K., Bloemen J., Brooks R. (2001): The growth of Bacillus stearothermophilus on stainless steel. Journal of Applied Microbiology, 90: 151-157. Go to original source... Go to PubMed...
    14. Gaillard S., Leguerinel I., Mafart P. (1998): Model for combined effects of temperature, pH, and water activity on thermal inactivation of Bacillus cereus spores. Journal of Food Science, 63: 887-889. Go to original source...
    15. Granum P.E. (1994): Bacillus cereus and its toxins. Journal of Applied Bacteriology, 76: 61-66. Go to original source...
    16. Granum P.E., Lund T. (1997): Bacillus cereus and its food poisoning toxins. FEMS Microbiology Letters, 157: 223-228. Go to original source... Go to PubMed...
    17. Granum P.E., Brynestad S., O'sullivan K., Nissen H. (1993): Enterotoxin from Bacillus cereus: Production and biochemical characterization. Bulletin of the IDF, 287: 38-41.
    18. Granum P.E, Thomas J.M., Alouf J.E. (1995): A survey of bacterial toxins involved in food poisoning: a suggestion for bacterial food poisoning toxin nomenclature. International Journal of Food Microbiology, 28: 129-144. Go to original source... Go to PubMed...
    19. Lake R., Hudson A., Cressey P. (2004): Risk profile: Bacillus spp. in rice. In: Client Report FW0319,FSANZ, 40.
    20. Lund T., Granum P.E. (1996): Characterization of a nonhaemolytic enterotoxin complex from Bacillus cereus isolated after a foodborne outbreak. FEMS Microbiology Letters, 141: 151-156. Go to original source... Go to PubMed...
    21. Marshall K.C (1984): Microbial Adhesion and Aggregation. Springer Verlag, Berlin. Go to original source...
    22. Marshall K.C. (1992): Biofilms: an overview of bacterial adhesion, activity, and control at surfaces. American Society for Microbiology, 58: 202-207.
    23. Notermans S., Batt C.A. (1998): A risk assessment approach for food-borne Bacillus cereus and its toxins. Journal of Applied Microbiology (Symposium Supplement), 84: 51-61. Go to original source... Go to PubMed...
    24. Phelps R.J., McKillip J.L. (2002): Enterotoxin production in natural isolates of Bacillaceae outside the Bacillus cereus group. Applied and Environmental Microbiology, 68: 3147-3151. Go to original source... Go to PubMed...
    25. Pipek P., Beneš P., Kvasnička F., Wilmink M. (1999): Mechanisch entbeintes Geflügelfleisch und dessen Haltbarkeit. Fleischerei-Technik, 15: 41-42.
    26. Pipek P., Houška M., Jeleníková J., Kýhos K., Hoke K., Šikulová M. (2005): Microbial decontamination of beef carcasses by combination of steaming and lactic acid spray. Journal of Food Engineering, 67: 309-315. Go to original source...
    27. Pirttijärvi T.S.M., Andersson M., Salkinoja-Salonen M.S. (2000): Properties of B. cereus and other bacilli contaminating biomaterial-based industrial processes. International Journal of Food Microbiology, 60: 231-239. Go to original source... Go to PubMed...
    28. Prüss B.M., Dietrich R., Nibler B., Märtlbauer E., Scherer S. (1999): The hemolytic enterotoxin HBL is broadly distributed among species of the Bacillus cereus group. Applied and Environmental Microbiology, 65: 5436-5442. Go to original source... Go to PubMed...
    29. Rusul g., Yaacob N.H. (1995): Prevalence of Bacillus cereus in selected foods and detection of enterotoxin using TECRA-VIA and BCET-RPLA. International Journal of Food Microbiology, 25: 131-139. Go to original source... Go to PubMed...
    30. Ryan P.A., Macmillan J.D., Zilinskas B.A. (1997): Molecular cloning and characterization of the genes encoding the L1 and L2 components of hemolysin BL from Bacillus cereus. Journal of Bacteriology, 179: 2551-2556. Go to original source... Go to PubMed...
    31. Svensson B., Eneroth A., Brendehaug J., Molin G., Christiansson A. (2000): Involvement of a pasteurizer in the contamination of milk by Bacillus cereus in a commercial dairy. Journal of Dairy Research, 67: 455-460. Go to original source... Go to PubMed...
    32. Te Gieffel M.C., Beumer R.R., Bonestroo M.H., Rombouts F.M. (1996): Incidence and characterization of Bacillus cereus in two dairy processing plants. Netherlands Milk and Dairy Journal, 50: 479-492.
    33. Thompson N.E., Keterhagen M.J., Bergdoll M.S., Schantz E.J. (1984): Isolation and some properties of an enterotoxin produced by Bacillus cereus. Infection and Immunity, 43: 887-94. Go to original source... Go to PubMed...
    34. Turnbul P.C.B. (2004): Bacillus spp. Availabe at: http://gsbs.utmb.edu/microbook/ch015.htm

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