Czech J. Food Sci., 2009, 27(3):194-202 | DOI: 10.17221/120/2008-CJFS

Distribution of non-tuberculosis mycobacteria in environmental samples from a slaughterhouse and in raw and processed meats

Monika Morávková, Marija Kaevska, Neysan Donnelly, Ivo PAVLÍK
Veterinary Research Institute, Brno, Czech Republic

The notification of all cases of diagnosed bovine tuberculosis is a statutory requirement, while the same is not true for other mycobacterial infections. Thus, the establishment of the true incidence of infection with non-tuberculous mycobacteria (NTM) is difficult. The aim of this study was to describe the incidence of NTM in environmental samples from a pig slaughterhouse and from raw and processed meat samples collected from supermarkets and butchers. Three species of mycobacteria (M. chelonae, M. kansasii, and M. intermedium) were detected in 8.0% of the environmental samples from a pig slaughterhouse and in 9.3% of raw and 7.7% of processed meat, respectively. The isolation of a single NTM species from these samples is a disturbing finding and means that raw meat may be a potential pathway for the transmission of NTM infections to humans.

Keywords: mycobacteria; meat; food safety; zoonosis

Published: June 30, 2009  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Morávková M, Kaevska M, Donnelly N, PAVLÍK I. Distribution of non-tuberculosis mycobacteria in environmental samples from a slaughterhouse and in raw and processed meats. Czech J. Food Sci. 2009;27(3):194-202. doi: 10.17221/120/2008-CJFS.
Download citation

References

  1. Abubakar I., Myhill D.J., Hart A.R., Lake I.R, Harvey I., Rhodes J.M., Robinson R., Lobo A.J., Probert C.S.J., Hunter P.R. (2007): A case-control study of drinking water and dairy products in Crohn's disease - further investigation of the possible role of Mycobacterium avium paratuberculosis. American Journal of Epidemiology, 165: 776-783. Go to original source... Go to PubMed...
  2. Arakawa C.K., Fryer J.L., Sanders J.E. (1986): Serology of Mycobacterium chelonae isolated from salmonid fish. Journal of Fish Diseases, 9: 269-271. Go to original source...
  3. Ayele W.Y., Svastova P., Roubal P., Bartos M., Pavlik I. (2005): Mycobacterium avium subsp. paratuberculosis cultured from locally and commercially pasteurized cow's milk in the Czech Republic. Journal of Applied and Environmental Microbiology, 71: 1210-1214. Go to original source... Go to PubMed...
  4. Cerf O., Griffiths M.W. (2000): Mycobacterium paratuberculosis heat resistance. Letters in Applied Microbiology, 30: 341-342. Go to original source... Go to PubMed...
  5. Chinabut S., Limsuwan C., Chanratchakool P. (1990): Mycobacteriosis in the snakehead, Channa striatus (Fowler). Journal of Fish Disease, 13: 531-535. Go to original source...
  6. Dunn B.L., Hodgson D.J. (1982): Atypical mycobacteria in milk. Journal of Applied Bacteriology, 52: 373-376. Go to original source... Go to PubMed...
  7. Dvorska L., Matlova L., Ayele W.Y., Fischer O.A., Amemori T., Weston J., Alvarez R.T., Beran V., Moravkova M., Pavlik I. (2007): Avian tuberculosis in naturally infected captive water birds of the Ardeidae and Threskiornithidae families studied by serotyping, IS901 RFLP typing and virulence for poultry. Veterinary Microbiology, 119: 366-374. Go to original source... Go to PubMed...
  8. Falkinham J.O. (2002): Non tuberculous mycobacteria in the environment. Journal of Clinics in Chest Medicine, 23: 529-551. Go to original source... Go to PubMed...
  9. Fischer O., Matlova L., Bartl J., Dvorska L., Melicharek I., Pavlik I. (2000): Findings of mycobacteria in insectivores and small rodents. Folia Microbiologica, 45: 147-152. Go to original source... Go to PubMed...
  10. Fischer O., Matlova L., Dvorska L., Svastova P., Bartl J., Melicharek I., Weston R.T., Pavlik I. (2001): Diptera as vectors of mycobacterial infections in cattle and pigs. Medical and Veterinary Entomology, 15: 208-211. Go to original source... Go to PubMed...
  11. Gebo K.A., Srinivasan A., Perl T.M., Ross T., Groth A., Merz W.G. (2002): Pseudo-outbreak of Mycobacterium fortuitum on a Human Immunodeficiency Virus Ward: transient respiratory tract colonization from a contaminated ice machine. Clinical Infectious Diseases, 35: 32-38. Go to original source... Go to PubMed...
  12. Gutierrez Garcia J.M. (2006): Meat as a vector of transmission of bovine tuberculosis to humans in Spain: a historical perspective. Veterinary Heritage, 29: 25-27.
  13. Hirose Y., Sugie S., Yoshimi N., Matsunaga K., Hara A., Mori H. (1998): Induction of apoptosis in colonic epithelium treated with 2-amino-1-methyl-6-phenylimidazol [4,5-b] pyridine (PhIP) and its modulation by a P4501A2 inducer, beta-naphthoflavone, in male F344 rats. Cancer Letters, 123: 167-172. Go to original source... Go to PubMed...
  14. Horsburgh C.R. (1991): Current concepts - Mycobacterium avium complex infection in the acquired immunodeficiency syndrome. The New England Journal of Medicine, 324: 1332-1338. Go to original source... Go to PubMed...
  15. Hruska K., Bartos M., Kralik P., Pavlik I. (2005): Mycobacterium avium subsp. paratuberculosis in powdered infant milk: paratuberculosis in cattle - the public health problem to be solved. Veterinarni Medicina, 50: 327-335. Go to original source...
  16. Iivanainen E., Martikainen P.J., Katila M.L. (1995). Effect of freezing of water samples on viable counts of environmental mycobacteria. Letters in Applied Microbiology, 21: 257-260. Go to original source... Go to PubMed...
  17. Ikonomopoulos J., Pavlik I., Bartos M., Svastova P., Ayele W.Y., Roubal P., Lukas J., Cook N., Gazouli M. (2005): Detection of Mycobacterium avium subsp. paratuberculosis in retail cheeses from Greece and the Czech Republic. Applied and Environmental Microbiology, 71: 8934-8936. Go to original source... Go to PubMed...
  18. Matlova L., Dvorska L., Ayele W.Y., Bartos M., Amemori T., Pavlik I. (2005): Distribution of Mycobactetium avium complex isolates in tissue samples of pigs fed peat naturally contaminated with mycobacteria as a supplement. Journal of Clinical Microbiology, 43: 1261-1268. Go to original source... Go to PubMed...
  19. Mediel M.J., Rodriguez V., Codina G., MartinCasabona N. (2000): Isolation of mycobacteria from frozen fish destined for human consumption. Journal of Applied and Environmental Microbiology, 66: 3637-3638. Go to original source... Go to PubMed...
  20. Merkal R.S., Whipple D.L. (1980): Inactivation of Mycobacterium bovis in meat products. Journal of Applied and Environmental Microbiology, 40: 282-284. Go to original source... Go to PubMed...
  21. Pavlik I., Svastova P., Bartl J., Dvorska L., Rychlik I. (2000): Relationship between IS901 in the Mycobacterium avium complex strains isolated from birds, animals, humans, and environment and virulence for poultry. Clinical and Diagnostic Laboratory Immunology, 7: 212-217 Go to original source... Go to PubMed...
  22. Pavlik I., Matlova L., Dvorska L., Shitaye J.E., Parmova I. (2005): Mycobacterial infections in cattle and pigs caused by Mycobacterium avium complex members and atypical mycobacteria in the Czech Republic during 2000-2004. Veterinarni Medicina, 50: 281-290. Go to original source...
  23. Pavlik I., Matlova L., Gilar M., Bartl J., Parmova I., Lysak F., Alexa M., Dvorska-Bartosova L., Svec V., Vrbas V., Horvathova A. (2007). Isolation of conditionally pathogenic mycobacteria from the environment of one pig farm and the effectiveness of preventive measures between 1997 and 2003. Veterinarni Medicina, 52: 392-404. Go to original source...
  24. Pedersen A., Reibel J. (1989): Intraoral infection with Mycobacterium chelonae. A case report. Oral Surgery, Oral Medicine, and Oral Pathology, 67: 262-265. Go to original source... Go to PubMed...
  25. Ristola M.A., von Reyn C.F., Arbeit R.D., Soini H., Lumio J., Ranki A., Buhler S., Waddell R., Tosteson A.N., Falkinham J.O., Sox C.H. (1999): High rates of disseminated infection due to non-tuberculous mycobacteria among AIDS patients in Finland. The Journal of Infection, 39: 61-67. Go to original source... Go to PubMed...
  26. Schulze RÖbbecke R., Fischeder R. (1989): Mycobacteria in biofilms. Zentralblatt für Hygiene und Umweltmedizin, 188: 385-390. Go to PubMed...
  27. Shitaye J.E., Getahun B., Alemayehu T., Skoric M., Treml F., Fictum P., Vrbas V., Pavlik I. (2006a): A prevalence study of bovine tuberculosis by using abattoir meat inspection and tuberculin skin testing data, histopathological and IS6110 PCR examination of tissues with tuberculous lesions in cattle in Ethiopia. Veterinarni Medicina, 51: 512-522. Go to original source...
  28. Shitaye J.E., Parmova I., Matlova L., Dvorska L., Horvathova A., Vrbas V., Pavlik I. (2006b): Mycobacterial and Rhodococcus equi infections in pigs in the Czech Republic between the years 1996 and 2004: the causal factors and distribution of infections in the tissues. Veterinarni Medicina, 51: 497-511. Go to original source...
  29. Shoda R., Matsueda K., Yamato S., Umeda N. (1996): Epidemiological analysis of Crohn's disease in Japan: increased dietary intake of n-6 polyunsaturated fatty acids and animal protein relates to the increased incidence of Crohn's disease in Japan. American Journal of Clinics and Nutrition, 63: 741-745. Go to original source... Go to PubMed...
  30. Swanson D.S., Pan X., Kline M.W., McKinney R.E. Jr., Yogev R., Lewis L.L., Brady M.T., McSherry G.D., Dankner W.M., Musser J.M. (1998): Genetic diversity among Mycobacterium avium complex strains recovered from children with and without human immunodeficiency virus infection. Journal of Infectious Disease, 178: 776-782. Go to original source... Go to PubMed...
  31. Teklu A., Asseged B., Yimer E., Gebeyehu M., Woldesenbet Z. (2004): Tuberculous lesions not detected by routine abattoir inspection: the experience of the Hossana municipal abattoir, southern Ethiopia. Revue Scientifique et Technique-Office International Des Epizooties, 23: 957-964. Go to original source... Go to PubMed...
  32. Tison F., Devulder B., Roos P., Tacquet A. (1966): Techniques et resultats de la recherche des mycobactéries dans les viandes. Annales de L'Institut Pasteur de Lille, 17: 155-160. Go to PubMed...
  33. Thomas S., McDurmont D.C. (1975): Isolation of acidfast organisms from milk and oysters. Health Laboratory Science, 12: 16-19. Go to original source... Go to PubMed...
  34. Wallace R.J. Jr. (1994): Recent changes in taxonomy and disease manifestations of the rapidly growing mycobacteria. European Journal of Clinical Microbiology and Infectious Disease, 13: 953-960. Go to original source... Go to PubMed...
  35. Wayne L.G., Kubica G.P. (1986): Family Mycobacteriaceae Chester 1896, 363AL. In: Sneath P.H.A., Mair N.S., Holt J.G. (eds): Bergey's Manual of Systematic Bacteriology. 2nd Part. 1st Ed. Williams and Wilkins, Baltimore: 1436-1457.
  36. Wilton S., Cousins D. (1992): Detection and identification of multiple mycobacterial pathogens by DNA amplification in a single tube. PCR Methods Applications, 1: 269-273. Go to original source... Go to PubMed...
  37. Yajko D.M., Chin D.P., Gonzalez P.C., Nassos P.S., Hopewell P.S., Reingold A.L., Horsburgh C.R. Jr., Yakrus M.A., Ostroff S.M., Hadley W.K. (1995): Mycobacterium avium complex in water, food, and soil samples collected from the environment of HIV-infected individuals. Journal of Acquired Immune Deficiency Syndrome and Human Retrovirology, 9: 176-182.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.