Czech J. Food Sci., 2009, 27(2):109-117 | DOI: 10.17221/1056-CJFS

Antioxidant activity of phenolic fractions of Mallotus philippinensis bark extract

Muhammad ARFAN1, Hazrat AMIN1, Magdalena KARAMAĆ2, Agnieszka KOSIŃSKA2, Wiesław WICZKOWSKI2, Ryszard AMAROWICZ2
1 Instutute of Chemical Sciences, University of Peshawar, Peshawar, Pakistan
2 Division of Food Science, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland

Phenolic compounds were extracted from Mallotus philippinensi bark using methanol. Six fractions (I-VI) were separated from the extract on a Sephadex LH-20 column using ethanol and acetone-water as the mobile phases and were evaluated for their total antioxidant activity, antiradical activity against DPPH* (2,2-diphenyl-1-picrylhydrazyl radical), and reducing power. The total phenolics and tannin contents in the fractions were determined. The content of total phenolics in the fractions ranged from 54 mg/g (fraction I) to 927 mg/g (fraction VI). Condensed tannins were detected in fractions II-VI. Total antioxidant activity (TAA) of phenolic fractions of Mallotus philipinensis bark extract ranged from 0.58 mmol Trolox/g (fraction I) to 6.82 mmol Trolox/g (fraction IV). Fraction IV also showed the strongest antiradical activity against DPPH* and reducing power. Several phenolic constituents in the fractions were detected by RP-HPLC using a gradient solvent system with UV-DAD detection.

Keywords: Mallotus philippinensis; bark; extract; natural antioxidants; phenolic compounds; tannins; antioxidant activity; antiradical activity

Published: April 30, 2009  Show citation

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ARFAN M, AMIN H, KARAMAĆ M, KOSIŃSKA A, WICZKOWSKI W, AMAROWICZ R. Antioxidant activity of phenolic fractions of Mallotus philippinensis bark extract. Czech J. Food Sci. 2009;27(2):109-117. doi: 10.17221/1056-CJFS.
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