Czech J. Food Sci., 2009, 27(2):102-108 | DOI: 10.17221/166/2008-CJFS

Microbiological, chemical, and sensory assessment of Pacific oysters (Crassostrea gigas) stored at different temperatures

Rong Cao1, Chang-Hu Xue1, Qi Liu2, Yong Xue1
1 College of Food Science and Engineering, Ocean university of China, Qingdao, Shandong Province, P. R. China
2 Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong Province, P. R. China

The changes were studied in microbiological, chemical, and sensory properties of Pacific oysters stored at 10°C, 5°C, and 0°C. Pseudomonas (22%) and Vibrionaceae (20%) species were dominant in raw oysters. The dominant bacteria found in the spoiled samples were Pseudomonas regardless of the storage temperature. During storage, rapid increases in aerobic plate count (APC) values of the samples stored at 10°C and 5°C were observed, while no obvious lag phases were detected. With the samples stored at 0°C, a decrease in APC value during the first 4 days and a lag phase of about 6 days were observed. The APC values of the samples stored at 10°C, 5°C, and 0°C reached the level of 107 CFU/g on day 6, 10, and 18, respectively. All the tested samples stored at different temperatures revealed a slight decrease in pH and a significant increase of total volatile basic nitrogen (TVB-N) during storage. The average TVB-N concentration of about 22.0 mg N/100 g was observed at the end of the shelf-life as determined by APC. Combined with the sensory assessments, the shelf-life of 6-7, 10-11, and 17-18 days for oysters stored at 10°C, 5°C, and 0°C, respectively, was determined.

Keywords: Pacific oyster; microbial flora; quality assessment; shelf-life

Published: April 30, 2009  Show citation

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Cao R, Xue C, Liu Q, Xue Y. Microbiological, chemical, and sensory assessment of Pacific oysters (Crassostrea gigas) stored at different temperatures. Czech J. Food Sci. 2009;27(2):102-108. doi: 10.17221/166/2008-CJFS.
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    References

    1. Ashie I.N.A., Smith J.P., Simpson B.K. (1996): Spoilage and shelf-life extension of fresh fish and shellfish. Critical Reviews in Food Science and Nutrition, 36: 87-121. Go to original source... Go to PubMed...
    2. Bagge-Ravn d., Ng y., Hjelm m., Christiansen J.N., Johansen C., Gram L. (2003): The microbial ecology of processing equipment in different fish industriesanalysis of the microflora during processing and following cleaning and disinfection. International Journal of Food Microbiology, 87: 242-43. Go to original source... Go to PubMed...
    3. Balasundari S.T., Jawahar S.A., Shanmugam G. (1997): Frozen storage performance of edible oyster Crassostrea madrasensis (Preston). Journal of Food Science and Technology, 34: 434-436.
    4. Banks A., Dawson J.A., Feiger E.A., Novak A.F., Poters J.A., Stavin J.W., Waterman J.J. (1977): Freezing of shellfish. In: Desrosier N.N., Tressler D.K. (eds): Fundamentals of Food Freezing. Avi, Westpon, Conn: 318-356. Go to original source...
    5. El-Marrakchi A., Bennour B., Bouchriti N., Hamama A., Tagafait H. (1990): Sensory, chemical, and microbiological assessments of moroccan sardines (Sardina pilchardus) stored in ice. Journal of Food Protection, 53: 600-605. Go to original source... Go to PubMed...
    6. Gennari M., Tomaselli S., Cotrona V. (1999): The microflora of fresh and spoiled sardines (Sardina pilchardus) caught in Adriatic (Mediterranean) Sea and stored in ice. Journal of Food Microbiology, 16: 15-28. Go to original source...
    7. Gerhardt P., Murray R.G.E., Costilow R.N., Nester E.W., Wood W.A., Krieg N.R., Philips G.B. (eds) (1981): Manual of Methods for General Bacteriology. American Society for Bacteriology, Washington: 1-525.
    8. Gill T.A. (1992): Chemical and biochemical indices in seafood quality. In: Huss H.H., Jacobsen M., Liston J. (eds): Quality Assurance in the Fish Industry. Elsevier Science Publishers, Amsterdam: 377-387.
    9. Gill T.A. (1997): Advanced analytical tools in seafood science. In: Luten J.B., Borensen T., Oehlenschlager J. (eds): Quality Assurance in the Fish Industry. Elsevier Science Publishers, Amsterdam: 479-490.
    10. Gram L., Huss H.H. (1996): Microbiological spoilage of fish and fish products. International Journal of Food Microbiology, 33: 121-137. Go to original source... Go to PubMed...
    11. Gram L., Huss H.H. (2000): Fresh and processed fish and shellfish. In: Lund B.M., Baird-Parker T.C., Gould G. W. (eds): The Microbiological Safety and Quality of Food. Vol. 1. Aspen Publishers Inc., Gaithersburg, Maryland: 472-506.
    12. Gram L., Melchiorsen J. (1996): Interaction between fish spoilage bacteria Pseudomonas spp. and Shewanella putrefaciens in fish extracts and on fish tissue. Journal of Applied Bacteriology, 80: 589-595. Go to original source... Go to PubMed...
    13. Harpaz S., Glatman L., Drabkin V., Gelman A. (2003): Effects of herbal essential oils used to extend the shelflife of freshwaterreared Asian sea bass fish (Lates calcarifer). Journal of Food Protection, 66: 410-417. Go to original source... Go to PubMed...
    14. He H., Morrissey M.T. (1999): Guide to Oyster Freshness Grades. Oregon State University Seafood Laboratory report. Astoria: 10.
    15. Holt J.G., Krieg N.R., Sneath P.H.A., Staley J.T., Wiilliams S.T. (1993): Bergey's Manual of Determinative Bacteriology. 9th Ed. Williams & Wilkins, Baltimore.
    16. Kim Y.-M., Paik H.-D., Lee D.-S. (2002): Shelf-life characteristics of fresh oysters and ground beef as affected by bacteriocin-coated plastic packaging film. Journal of the Science of Food & Agriculture, 82: 998-1002. Go to original source...
    17. Koutsoumanis K., Lambropoulou K., Nychas G.J.E. (1999): Biogenic amines and sensory changes associated with the microbialflora of Mediterranean gilt-head sea bream (Sparus aurata) stored aerobically at 0, 8, and 15°C. Journal of Food Protection, 62: 398-402. Go to original source... Go to PubMed...
    18. Lannelongue M., Finne G., Hanna M.O., Nickelson R., Vanderzani C. (1982): Microbiological and chemical changes during storage of swordfish (Xiphias gladius) steaks in retail packages containing CO2-enriched atmospheres. Journal of Food Protection, 45: 1197-1203. Go to original source... Go to PubMed...
    19. Ortigosa M., Garay E., Pujalte M.J. (1994): Numerical taxonomy of aerobic, Gram-negative bacteria associated with oysters and surrounding seawater of the Mediterranean coast. Systematic & Applied Microbiology, 17 (4): 589-600. Go to original source...
    20. Puchenkova S.G. (1991): Sanitary-microbiological investigation of oysters and water from the North Caucasus region of the Black Sea. Gigiena i Sanitariia, 3: 22-24.
    21. Reineccius G. (1990): Off-flavors in foods. Critical Reviews in Food Science and Nutrition, 29: 381-402. Go to original source... Go to PubMed...
    22. Ruiz-Capillas C., Moral A. (2005): Sensory and biochemical aspects of quality of whole bigeye tuna (Thunnus obesus) during bulk storage in controlled atmospheres. Food Chemistry, 89 (3): 347-354. Go to original source...
    23. Ryder J.M., Fletcher G.C., Stec M.G., Seelye R.J. (1993): Sensory, microbiological and chemical changes in hoki stored in ice. International Journal of Food Science and Technology, 28: 169-180. Go to original source...
    24. Shewan J.M. (1971): The microbiology of fish and fishery products. Journal of Applied Bacteriology, 34: 299-315. Go to original source... Go to PubMed...
    25. Shewan J.M. (1977): The bacteriology of fresh and spoiling fish and the biochemical changes induced by bacterial action. In: Proceedings of a Conference on Handling, Processing and Marketing of Tropical Fish. Tropical Products Institute, London: 51-66.
    26. Ward D.R., Baj N.J. (1988): Factors affecting microbiological quality of seafood. Food Technology, 3: 85-89.

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