Czech J. Food Sci., 2009, 27(1):49-54 | DOI: 10.17221/134/2008-CJFS

Molecular cloning and characterisation of alpha subunit of H+-ATPase in Lactobacillus casei Zhang

Xia Chen1, Mei Yang1, Zhihong Sun1, Wenjun Liu1, Tiansong Sun1, He Meng2, Heping Zhang1
1 Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Huhhot, P.R.China
2 School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P.R.China

The acid tolerance is an important property of Lactic acid bacteria as potential probiotics. H+-ATPase is considered a key gene in several bacteria with the ability of acid tolerance. We cloned and sequenced the full length cDNA of alpha subunit of H+-ATPase gene in Lactobacillus casei Zhang, which had been isolated from traditional home-made koumiss in Inner Mongolia of China. The results showed that the respective cDNA sequence is composed of 1530 nucleotides and codes a putative protein including 509 amino acids. In addition, we also reconstructed the phylogenic trees for H+-ATPase gene based on amino acids sequences of diverse strains of Lactic acid bacteria.

Keywords: H+-ATPase; clone; sequence; Lactobacillus casei Zhang; phylogenic trees

Published: February 28, 2009  Show citation

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Chen X, Yang M, Sun Z, Liu W, Sun T, Meng H, Zhang H. Molecular cloning and characterisation of alpha subunit of H+-ATPase in Lactobacillus casei Zhang. Czech J. Food Sci. 2009;27(1):49-54. doi: 10.17221/134/2008-CJFS.
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