Czech J. Food Sci., 2008, 26(10):S25-S32 | DOI: 10.17221/246/2008-CJFS

Relations between polyphenols content and antioxidant activity in vine grapes and leaves

Josef BALÍK¹, Marie KYSELÁKOVÁ², Naděžda VRCHOTOVÁ³, Jan TŘÍSKA³, Michal KUMŠTA², Jaromír VEVERKA², Pavel HÍC¹, Jiří TOTUŠEK⁴, Danuše LEFNEROVÁ⁴

¹ Department of Post-harvest Technology of Horticultural Products and

² Department of Viticulture and Oenology, Faculty of Horticulture, Mendel University of Agriculture and Forestry in Brno, Lednice, Czech Republic

³ Institute of Systems Biology and Ecology, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic

⁴ Institute of Preventive Medicine, Medical Faculty, Masaryk University, Brno, Czech Republic$2

The occurrence and content of some polyphenols and the antioxidant activity of compounds present in grape berries, stems and leaves of Vitis vinifera L. were evaluated. Three white and three blue varieties of grapevine were investigated. The contents were determined of trans-resveratrol, trans-piceid, caftaric acid, tryptophan, catechin, epicatechin, total polyphenols, and flavanols, both in healthy material and in the samples of the plant material infested with microorganisms (Botryotinia fuckeliana Whetzel anamorph Botrytis cinerea Pers.; Uncinula necator (Schw.) Burr; Plasmopara viticola (Berk. & M.A. Curtis) Berl & De Toni). The antioxidant activity of the extracts obtained was determined by different methods: FRAP (Ferric Reducing Antioxidant Power), DPPH (2,2-diphenyl-1-picrylhydrazyl radical) and TAC-PCL (Total Antioxidant Capacity of Photochemiluminescence). The content of trans-resveratrol varied between 0.3-2.3 mg/kg and 0.7-12.1 mg/kg in non-infested and infested grape berries, respectively. The content of trans-piceid between 0.6-2.9 mg/kg and 1.5-6.3 mg/kg in non-infested and infested grape berries, respectively. The content of trans-resveratrol varied between 2.5-10.3 mg/kg and 3.7-20.9 mg/kg in healthy and in infected leaves, respectively. The content trans-piceid varied between 11.3-58.4 mg/kg and 18.5-60.9 mg/kg in the healthy and in the infected leaves, respectively. The highest content of trans-resveratrol was found in stems (16.3-276.3 mg/kg). In young lateral shoots, the highest levels of trans-piceid (12.6-99.7 mg/kg) and caftaric acid (474-2257 mg/kg) were determined. The infested grape berries showed a higher antioxidant activity, which was most closely correlated with the content of total polyphenols (correlation coefficient = 0.8336-0.9952).

Keywords: vine grapes; vine leaves; stems; downy mildew; powdery mildew; grey mould; piceid; resveratrol; caftaric acid; catechin; epicatechin; photochemiluminescence; DPPH; FRAP

Published: December 31, 2008  Show citation