Czech J. Food Sci., 2008, 26(6):458-463 | DOI: 10.17221/14/2008-CJFS

FT-IR spectroscopic characteristics of differently cultivated Escherichia coli

Zdenek FILIP1,2,*, SUSANNE HERMANN1,*, KATEŘINA DEMNEROVÁ2
1 Federal Environmental Agency, Langen Branch, Langen, Germany
2 Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic

FT-IR spectra were recorded of Escherichia coli cell mass with the aim of obtaining spectral traits possibly useful in a rapid detection and characterisation of this indicator bacterium. A well differentiated spectrum was obtained from the cell mass harvested in a stationary phase of growth, e.g., after 24 h, from a minimum nutrient broth. The cell mass, harvested either earlier or grown in nutrient solutions which contained an enhanced carbon or nitrogen concentrations delivered somewhat different IR spectra, apparently due to a higher content of nucleic acid components as related to other structural constituents of bacterial cells. Consequently, the FT-IR spectra of E. coli, although rather rapidly to collect, seem only capable of delivering useful and reproducible information if the cell mass is obtained under standardised cultural conditions.

Keywords: Escherichia coli; FT-IR spectroscopy; cell mass traits

Published: December 31, 2008  Show citation

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FILIP Z, HERMANN S, DEMNEROVÁ K. FT-IR spectroscopic characteristics of differently cultivated Escherichia coli. Czech J. Food Sci. 2008;26(6):458-463. doi: 10.17221/14/2008-CJFS.
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    References

    1. Dufour A.P. (1977): Escherichia coli: The fecal coliform. In: Hoadley A.W., Dutka B.J.: Bacterial Indicators/Health Hazards Associated with Water. American Society for Testing and Materials, Philadelphia: 48-58. Go to original source...
    2. Edberg S.c., Allen M.j., Smith D.b. (1991): Rapid, specific, defined substrate technology colilert system for the simultaneous detection of total coliforms and Escherichia coli from water. In: Vaheri A., Tilton R.C., Balows A.: Rapid Methods and Automation in Microbiology and Immunology. Springer, Berlin: 436-443. Go to original source...
    3. Filip Z. (1978): Infrared spectroscopy of two soils and their components. In: Krumbein W.E. (ed.): Environmental Biogeochemistry and Geomicrobiology. Volume 3. Methods, Metals and Assessment. Ann Arbor Science Publishers, Ann Arbor, Michigan: 747-752.
    4. Filip Z., Hermann S. (2001): An attemp to differentiate Pseudomonas spp. and other soil bacteria by FT-IR spectroscopy. European Journal of Soil Biology, 37: 137-143. Go to original source...
    5. Filip Z., Hermann S., Kubat J. (2004): FT-IR spectroscopic characteristics of differently cultivated Bacillus subtilis. Microbiological Research, 159: 257-262. Go to original source... Go to PubMed...
    6. Gray G.W., Wilkinson S.G. (1965): The effect of ethylendiamintetra-acetic acids on the cell wall of some Gram-negative bacteria. Journal of General Microbiology, 39: 385-399. Go to original source... Go to PubMed...
    7. Johnston S.F. (1991): Fourier Transform Infrared - A Constantly Evolving Technology. Horwood, New York.
    8. Káš V. (1964): Zemědělská mikrobiologie. SZN, Praha.
    9. Káš V. (1966): Mikroorganismen im Boden. Ziemsen, Wittenberg-Lutherstadt.
    10. Kelly M.T., Renner D.J., Farmer III J.J. (1985): Enterobacteriaceae. In: Lannette E.H., Spaulding E.H., Truant J.P. (eds): Manual of Clinical Microbiology. American Society for Microbiology, Washington: 263-277.
    11. Koch A.L. (1979): Microbial growth in low concentrations of nutrients. Strategies of Microbial Life in Extreme Environments. VCH-Weinheim: 261-279. Go to original source...
    12. Kolter R. (1995): Survival in stationary phase. Microbes, Environment, Biotechnologies. Institut Louis Malarde, Papeete, Tahiti: 121-123.
    13. Kotrba P., Pospíšil P., Lorenzo De V., Ruml T. (1999): Enhanced metallosorption of Escherichia coli cells due to surface display of β- and α-domains of mammalian methalothionein as a fusion to lamb protein. Journal of Receptor & Signal Transduction Research, 19: 703-715. Go to original source... Go to PubMed...
    14. Kott Y. (1977): Current concepts of indiator bacteria. In: Hoadley A.W., Dutka B.J.: Bacterial Indicators/Health Hazards Associated with Water. American Society for Testing and Materials, Philadelphia: 3-14.
    15. Leadbetter E.R. (2002): Procaryotic diversity: Form, ecophysiology, and habitat. Manual of Environmental Microbiology. American Society for Microbiology Press, Washington: 19-31.
    16. Moe Ch.L. (2002): Waterborne transmission of infectious agents. In: Hurst Ch.J., Crawford R.L., Knudsen G.R., McInerney M.J., Stetzenbach L.D.: Manual of Environmental Microbiology. American Society of Microbiology Press, Washington: 184-204.
    17. Morisaki H. (1982): The electric current from Escherichia coli and the effect of resin on it. Journal of General and Applied Microbiology, 28: 73-86. Go to original source...
    18. Morisaki H. (1984): Effect of a liquid-liquid interface on the metabolic activity of Escherichia coli. Journal of General and Applied Microbiology, 30: 35-42. Go to original source...
    19. Naumann D. (2000): Infrared spectroscopy in microbiology. In: Meyers R.A. (ed.): Encyclopedia of Analytical Chemistry. Wiley, Chichester: 1-29. Go to original source...
    20. Neidhardt F.C. (1995): Adaptation of Escherichia coli to temperature extremes. Microbes, Environment, Biotechnologies. Institut Louis Malarde, Papeete, Tahiti: 117-120.
    21. Parker F.S. (1971): Infrared Spectroscopy in Biochemistry, Biology and Medicine. Hilger, London. Go to original source...
    22. Randle C., Albro P.W., Dittmer J.C. (1969): The phosphoglyceride composition of gram-negative bacteria and the changes in composition during growth. Biochimica et Biophysica Acta, 187: 214-220. Go to original source... Go to PubMed...
    23. Rose A.H. (1965): Chemical Microbiology. Butterworths, London.
    24. Smibert R.M., Krieg N.R. (1981): General characterization. In: Gerhardt P. (ed.): Manual of Methods for General Bacteriology. American Society for Microbiology, Washington: 409-443.
    25. Sousa C., Kotrba P., Ruml T., Cebolla A., Lorenzo De V. (1998): Metalloadsorption by Escherichia coli cells displaying yeast and mammalian metallothioneins anchored to the outer membrane protein lamB. Journal of Bacteriology, 180: 2280-2284. Go to original source... Go to PubMed...
    26. Wheater D.W., Mara D.D., Jawad L., Oragui J. (1980): Pseudomonas aeruginosa and Escherichia coli in sewage and fresh water. Water Research, 14: 713-721. Go to original source...

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