Czech J. Food Sci., 2008, 26(5):347-359 | DOI: 10.17221/27/2008-CJFS

Patatin, the major protein of potato (Solanum tuberosum L.) tubers, and its occurrence as genotype effect: processing versus table potatoes

Jan BÁRTA, Veronika BÁRTOVÁ
Department of Plant Production, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic

Patatin relative abundance in SDS-extractable protein and patatin content in dry matter were evaluated in tubers of forty processing and table potato cultivars usually cultivated in the Czech Republic, Germany, and the Netherlands. The patatin characteristics were evaluated over three experimental years. Patatin relative abundance in the processing cultivars achieved on average a significantly higher value (P < 0.001; Tukey HSD test) than patatin relative abundance in the table cultivars, resulting in average values of 25.80% and 21.59%, respectively. A high patatin relative abundance (over 30% in extractable protein) was determined only in the case of two cultivars: Vaneda (average 31.29%) and Tomensa (average 31.24%). Patatin content in tuber dry matter was significantly higher in the processing potato cultivars in all three experimental years (P < 0.001), attaining a mean of 1.28% with the processing cultivars and 1.03% with the table cultivars. The direct effect of the cultivar on patatin relative abundance in SDS-extractable protein was higher (33.1% for processing potato cultivars and 48.1% for table potato cultivars) than the effect of the growing year (15.6% for processing potato cultivar and 22.8% for table potato cultivars).

Keywords: Solanum tuberosum L.; potato tuber proteins; patatin; patatin relative abundance; patatin content

Published: October 31, 2008  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
BÁRTA J, BÁRTOVÁ V. Patatin, the major protein of potato (Solanum tuberosum L.) tubers, and its occurrence as genotype effect: processing versus table potatoes. Czech J. Food Sci. 2008;26(5):347-359. doi: 10.17221/27/2008-CJFS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Åhman I., Melander M. (2003): Potato proteins, and other plant proteins, as potential transgenic resistance factors to pollen beetles in oilseed rape. Annals of Applied Biology, 143: 253-260. Go to original source...
    2. Andrews D.L., Beames B., Summers M.D., Park W.D. (1988): Characterization of the lipid acyl hydrolase activity of the major potato (Solanum tuberosum) tuber protein, patatin, by cloning and abundant expression in a baculovirus vector. Biochemical Journal, 252: 199-206. Go to original source... Go to PubMed...
    3. Bánfalvi Z., Kostyál Z., Barta E. (1994): Solanum brevidens possesses a non-sucrose-inducible patatin gene. Molecular and General Genetics, 245: 517-522. Go to original source... Go to PubMed...
    4. Bárta J., Čurn V. (2004): Potato (Solanum tuberosum L.) tuber proteins - classification, characterization, importance. Chemické Listy, 98: 373-378.
    5. Bauw G., Nielsen H.V., Emmersen J., Nielsen K.L., Jorgensen M., Welinder K.G. (2006): Patatins, Kunitz protease inhibitors and other major proteins in tuber of potato cv. Kuras. FEBS Journal, 273: 3569-3584. Go to original source... Go to PubMed...
    6. Bradshaw J.E., Mackay G.R. (eds) (1994): Potato Genetics. CAB International, Walingford. Go to original source...
    7. Debre F., Brindza J. (1996): Genotypy zemiakov z pohµadu produkcie a úľitkovej hodnoty. Rostlinná Výroba, 42: 509-515.
    8. Hames B.D., Rickwood D. (eds) (1987): Gel Electrophoresis of Proteins. A Practical Approach. IRL Press Limited, Oxford.
    9. Hannapel D.J. (1991): Distribution of potato tuber proteins during development. American Potato Journal, 68: 179-190. Go to original source...
    10. Hunnius W., Fritz A., Munzert M. (1976): On the effect of year and course of weather on protein content of potatoes. Landwirtschaftliche Forschung, 29: 141-148. Go to original source...
    11. Jimenez M., Escribano J., Perez-Gilabert M., Chazarra S., Cabanes J., Garcia Carmona F. (2001): An octaethylene glycol monododecyl ether-based mixed micellar assay for determining the lipid acyl hydrolase activity of patatin. Lipids, 36: 1169-1174. Go to original source... Go to PubMed...
    12. Jørgenson M., Bauw G., Welinder K.G. (2006): Molecular properties and activities of tuber proteins from starch potato cv. Kuras. Journal of Agricultural and Food Chemistry, 54: 9389-9397. Go to original source... Go to PubMed...
    13. Koningsveld van, G.A., Gruppen H., Jongh de H.H.J., Boekel van M.A.J.S., Walstra P., Voragen A.G.J. (2001): The solubility of potato proteins from industrial potato fruit juice as influenced by pH and various additives. Journal of the Science of Food and Agriculture, 82: 134-142. Go to original source...
    14. Koningsveld van G.A., Walstra P., Voragen A.G.J., Kuijpers I.J., Boekel van M.A.J.S., Gruppen H.(2006): Effect of protein composition and enzymatic activity on formation and properties of potato protein stabilized emulsions. Journal of Agricultural and Food Chemistry, 54: 6419-6427. Go to original source... Go to PubMed...
    15. Kumar G.N.M., Houtz R.L., Knowles N.R. (1999): Age-induced protein modifications and increased proteolysis in potato seed-tubers. Plant Physiology, 119: 89-99. Go to original source... Go to PubMed...
    16. Lachman J., Hamouz K., Dvořák P., Orsák M. (2005): The effect of selected factors on the content of protein and nitrates in potato tubers. Plant, Soil and Environment, 51: 431-438. Go to original source...
    17. Lee L., Hannapel D., Mignery G., Shumway J., Park W. (1983): Control of tuber protein synthesis in potato. In: Goldberg R.B. (ed.): Plant Molecular Biology. UCLA Symposium, Alan R. Liss, New York: 355-365.
    18. Leszkowiat M.J., Yada R.Y., Coffin R.H., Stanley D.W., McKeown A.W. (1991): Free amino compound total nitrogen and dry matter content of summer potatoes during growth in southern Ontario. Canadian Institute of Food Science and Technology Journal, 24: 68-73. Go to original source...
    19. Lyn L., Youling L.X. (2005): Inhibition of lipid oxidation in cooked beef patties by hydrolyzed potato proteins is related to its reducing and radical scavenging ability. Journal of Agricultural and Food Chemistry, 53: 9186-9192. Go to original source... Go to PubMed...
    20. Macrae A.R., Visicchion J.E., Lanot A. (1998): Application of potato lipid acyl hydrolase for the synthesis of monoacylglycerols. Journal of the American Oil Chemists' Society, 75: 1489-1494. Go to original source...
    21. Mitrus J., Stankiewitz C., Steé E., Kamecki M., Starczewski J. (2003): The influence of selected cultivation on the content of total protein and amino acids in the potato tubers. Plant, Soil and Environment, 49: 131-134. Go to original source...
    22. Paiva E., Lister R.M., Park W.D. (1983): Induction and accumulation of major tuber proteins of potato in stems and petioles. Plant Physiology, 71: 161-168. Go to original source... Go to PubMed...
    23. Peyer C., Boney P., Staudacher E. (2004): Purification and characterization of ß-xylosidase from potatoes (Solanum tuberosum). BBA-Proteins and Proteomics, 1672: 27-35. Go to original source... Go to PubMed...
    24. Pots A.M. (1999): Physico-chemical properties and thermal aggregation of patatin, the major potato tuber protein. [PhD Thesis.] Wageningen Agricultural University, Wageningen.
    25. Pots A.M., Gruppen H., Diepenbeek van R., Lee van der J.J., Boekel van M.A.J.S., Wijngaards G., Voragen A.G.J. (1999): The effect of storage of whole potatoes of three cultivars on the patatin and protease inhibitor content; a study using capillary electrophoresis and MALDI-TOF mass spectrometry. Journal of the Science of Food and Agriculture, 79: 1557-1564. Go to original source...
    26. Prat S., Frommer W.B., Höfgen R., Keil M., Kossmann J., Köster-Töpfer M., Liu X.J., Müller B., Peña-Cortés H., Rocha-Sosa M. (1990): Gene expression during tuber development in potato plants. FEBS Letters, 268: 334-338. Go to original source... Go to PubMed...
    27. Racusen D. (1983): Occurrence of patatin during growth and storage of potato tubers. Canadian Journal of Botany, 61: 370-373. Go to original source...
    28. Racusen D., Foote M. (1980): A major soluble glycoprotein of potato. Journal of Food Biochemistry, 4: 43-52. Go to original source...
    29. Ralet M.-Ch., Guéguen J. (2000): Foaming properties of potato raw proteins and isolated fractions. Lebensmittel Wissenschaft und Technologie, 34: 266-269. Go to original source...
    30. Rajapakse D.P., Imai T., Ishige T. (1991): Analysis of potato microtuber proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Potato Research, 34: 285-293. Go to original source...
    31. Rosahl S., Schmidt R., Schell J., Willmitzer L. (1986): Isolation and characterization of a gene from Solanum tuberosum encoding patatin, the major storage protein of potato tubers. Molecular and General Genetics, 203: 214-220. Go to original source...
    32. Senda K., Yoshioka H., Doke N., Kawakita K. (1996): A cytosolic phospholipase A2 from potato tissues appears to be patatin. Plant and Cell Physiology, 37: 347-353. Go to original source... Go to PubMed...
    33. Shewry P.R. (2003): Tuber storage protein. Annals of Botany, 91: 755-769. Go to original source... Go to PubMed...
    34. Snyder J.C., Desborough S.L. (1978): Rapid estimation of potato tuber total protein content with Coomassie Brilliant Blue G-250. Theoretical and Applied Genetics, 52: 135-139. Go to original source... Go to PubMed...
    35. Straetkvern K.O., Schwarz J.G., Wiesenborn D.P., Zafirakos E., Lihme A. (1999): Expanded bed adsorption for recovery of patatin from crude potato juice. Bioseparation, 7: 333-345. Go to original source...
    36. Strickland J.A., Orr G.L., Walsh T.A. (1995): Inhibition of Diabrotical larval growth by patatin, the lipid acyl hydrolase from potato tubers. Plant Physiology, 109: 667-674. Go to original source... Go to PubMed...
    37. Tonón C., Daleo G., Oliva C. (2001): An acidic beta1,3 glucanase from potato tubers appears to be patatin. Plant Physiology and Biochemistry, 39: 849-854. Go to original source...
    38. Twell D., Ooms G. (1988): Structural diversity of the patatin family in potato cv. Désirée. Molecular and General Genetics, 212: 325-336. Go to original source... Go to PubMed...
    39. Wang L.L., Xiong Y.L. (2005): Inhibition of lipid oxidation in cooked beef patties by hydrolyzed potato protein is related to its reducing and radical scavenging ability. Journal of Agricultural and Food Chemistry, 53: 9186-9192. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content