Czech J. Food Sci., 2008, 26(4):291-297 | DOI: 10.17221/8/2008-CJFS

Optimisation of the PCR method for the detection of Campylobacter jejuni and Campylobacter coli in samples of ready-to-eat chicken meals

Zdeňka Šabatková1,2, Kateřina Demnerová1, Jarmila Pazlarová1
1 Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic
2 Department of Biomagnetic Techniques, Institute of Systems Biology and Ecology, Academy of Science of the Czech Republic, České Budějovice, Czech Republic

This work compared the use of polymerase chain reaction (PCR) and the conventional CSN/ISO/10272 culture-based methods in the detection of Campylobacter species in ready-to-eat meals made from chicken meat. PCR was carried out with the primers specific to C. jejuni, C. coli, C. lari, and was modified with an internal control. The detection of campylobacters by PCR was performed on both untreated and spiked samples of real food purchased in local stores. For PCR, the detection limit was 2 CFU/g after 48 h enrichment in Park and Sanders broth. Duplex PCR proved to be highly reliable in the detection of campylobacters in different food types. Without extra spiking, samples from a global fast food chain exhibited positive amplification of the PCR product while but negative results were obtained from the cultivation of the same samples.

Keywords: polymerase chain reaction; internal control; Campylobacter spp.

Published: August 31, 2008  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Šabatková Z, Demnerová K, Pazlarová J. Optimisation of the PCR method for the detection of Campylobacter jejuni and Campylobacter coli in samples of ready-to-eat chicken meals. Czech J. Food Sci. 2008;26(4):291-297. doi: 10.17221/8/2008-CJFS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Chuma T., Yano K., Omori K., Yugi H. (1997): Direct detection of Campylobacter jejuni in chicken cecal contents by PCR. Journal of Veterinary Medical Science, 59: 85-87. Go to original source... Go to PubMed...
    2. CSN ISO 10272 Microbiology of food and animal feeding stuffs - Horizontal method for detection of thermotolerant Campylobacter.
    3. Cubero J., Martinez M.C., Llop P., Lopez M.M. (1999): A simple and efficient PCR method for the detection of Agrobacterium tumefaciens in plant tumours. Journal of Applied Microbiology, 86: 591-602. Go to original source... Go to PubMed...
    4. Cubero J., van der Wolf J., van Beckhoven J., Lopt M.M. (2002): An internal control for the diagnosis of crown gall by PCR. Journal of Microbiological Methods, 51: 387-392. Go to original source... Go to PubMed...
    5. Fermer C., Engvall E.O. (1999): Specific PCR identification and differentiation of the thermophilic campylobacters, Campylobacter jejuni, C. coli, C. lari and C. upsaliensis. Journal of Clinical Microbiology, 37: 3370-3373. Go to original source... Go to PubMed...
    6. Friedman C.R., Neimann J., Wegener H.C., Tauxe R.V. (2000): Epidemiology of Campylobacter jejuni infections in the United States and other industrialized nations. In: Nachamkin I., Blaser M.J. (eds): Campylobacter. 2nd Ed. ASM Press, Washington: 121-138.
    7. Glunder G., Neumann U., Braune S. (1992): Occurrence of Campylobacter spp. in young gulls, duration of Campylobacter infection and reinfection by contact. Journal of Veterinary Medicine series B - Infectious Diseases and Veterinary Public Health, 39: 119-122. Go to original source... Go to PubMed...
    8. Linton D., Lawson A.J., Owen R.J., Stanley J. (1997): PCR detection, identification to species level, and fingerprinting of Campylobacter jejuni and Campylobacter coli direct from diarrheic samples. Journal of Clinical Microbiology, 35: 2568-2572. Go to original source... Go to PubMed...
    9. Lübeck P.S., Cook N., Wagner M., Fach P., Hoorfar J. (2003a): Towards an international standard for PCR-based detection of food-borne thermotolerant campylobacters: Validation in a multicenter collaborative trial. Applied Environmental Microbiology, 69: 5670-5672. Go to original source... Go to PubMed...
    10. Lübeck P.S., Wolffs P., On S.L.W., Agens P., Radström P., Hoorfar J. (2003b): Towards an international standard for PCR-based detection of food-borne thermotolerant campylobacters: Assay development and analytical validation. Applied Environmental Microbiology, 69: 5664-5669. Go to original source... Go to PubMed...
    11. Magistrado P.A., Garcia M.M., Raymundo A.K. (2001): Isolation and polymerase chain reaction-based detection of Campylobacter jejuni and Campylobacter coli from poultry in the Philippines. International Journal of Food Microbiology, 70: 197-206. Go to original source... Go to PubMed...
    12. Mead P.S., Slutsker L., Dietz V., McCaig L.F., Bresee J.S., Shapiro C., Griffin P.M., Tauxe R.V. (1999): Food-related illness and death in the United States. Emergency Infectious Diseases, 5: 607-625. Go to original source... Go to PubMed...
    13. Mohran Z.S., Arthur R.R., Oyofo B.A., Peruski L.F., Wasfy M.O., Ismail T.F., Murphy J.R. (1998): Differentiation of Campylobacter isolates on the basis of sensitivity to boiling in water as measured by PCRdetectable DNA. Applied Environmental Microbiology, 64: 363-365. Go to original source... Go to PubMed...
    14. Sabatkova Z., Pazlarova J., Demnerova K. (2004): Sample processing effect on polymerase chain reaction used for identification of Campylobacter jejuni. Folia Microbiologica, 49: 693-697. Go to original source... Go to PubMed...
    15. Sachadyn P., Kur J. (1998): The construction and use of PCR internal control. Molecular and Cellular Probes, 12: 259-262. Go to original source... Go to PubMed...
    16. Volokhov D., Chizhikov V., Chumakov K., Rasooly A. (2003): Microarray-based identification of thermophilic Campylobacter jejuni, C. coli, C. lari and C. upsaliensis. Journal of Clinical Microbiology, 41: 4071-4080. Go to original source... Go to PubMed...
    17. Winters D.K., Slavik M.F. (1995): Evaluation of a PCR based assay for specific detection of Campylobacter jejuni in chicken washes. Molecular and Cellular Probes, 9: 307-310. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content