Czech J. Food Sci., 2008, 26(4):254-258 | DOI: 10.17221/1120-CJFS

Efficient extraction of caffeic acid derivatives from adventitious roots of Echinacea purpurea

Chun-Hua Wu1, Hosakate N. Murthy1, 2, Eun-Joo Hahn1, Hyung L. Lee3, Kee-Yoeup Paek1
1 Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, South Korea
2 Department of Botany, Karnatak University, Dharwad, India
3 Deparment of Urology, College of Medicine, Kyung Hee University, Seoul, South Korea

Echinacea species are popular herbal medicine and food supplements for enhancing the immune system. This study was conducted with the aim of developing an efficient heat reflux extraction of caffeic acid derivatives from dried adventitious roots of Echinacea purpurea obtained in bioreactor cultures. Water, methanol (20, 40, 60, 80, and 100%), and ethanol (20, 40, 60, 80, and 100%) were used as solvents for the extraction of caffeic acid derivatives. Another parameter used for the optimisation was the solvent temperature during extraction. The treatment of samples with 60% ethanol at 60°C for 2 h proved to be the most suitable procedure. This treatment was also responsible for the higher yields of total phenolics, flavonoids, and polysaccharides.

Keywords: adventitious roots; caftaric acid; chlorogenic acid; chichoric acid; Echinacea purpurea

Published: August 31, 2008  Show citation

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Wu C, Murthy HN, Hahn E, Lee HL, Paek K. Efficient extraction of caffeic acid derivatives from adventitious roots of Echinacea purpurea. Czech J. Food Sci. 2008;26(4):254-258. doi: 10.17221/1120-CJFS.
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