Czech J. Food Sci., 2008, 26(4):242-253 | DOI: 10.17221/1119-CJFS

Comparison of the phenolic content and total antioxidant activity in wines as determined by spectrophotometric methods

Pavel STRATIL, Vlastimil KUBÁŇ, Jitka FOJTOVÁ
Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry in Brno, Brno, Czech Republic

Folin-Ciocalteu reagent (FCM) and Price and Butler method (PBM) were used for spectrophotometric determination of the total content of phenolic compounds in 29 wines (8 white, 21 red). The average contents of phenolic compounds determined by FCM and PBM were 108 (90-119) and 105 (90-129) for white wines, and 1545 (874-2262) and 547 (306-816) mg/l of gallic acid equivalents (GAE) for red wines, respectively. The reason for the lower PBM values in red wines is the higher reactivity in PBM of phenolic compounds, especially of gallic acid generally used as a standard in the above methods. The higher reactivity of the standard means that the measured absorbance of the sample responds to a lower concentration. The average total antioxidant activities determined by TEAC (Trolox Equivalent Antioxidant Capacity), FRAP (Ferric Reducing Antioxidant Power), and DPPH (using diphenyl-p-picrylhydrazyl radical) were 5.14 (4.30-6.14), 1.43 (0.86-2.14), and 0.71 (0.61-0.81) of Trolox equivalents (TE) and 26.44 (13. 9-34.4), 9.43 (4.92-13.9), and 5.52 (2.91-8.62) mmol/l TE for white and red wines, respectively. Almost the same molar absorptivities with TEAC and DPPH methods were found while with FRAP method it was somewhat higher (about 1.56-times). The ratio of the values determined by FRAP and DPPH methods for white and red wines were 2.0 and 1.7, respectively. The TEAC values were 2.8- and 4.8-fold higher than those determined by FRAP and DPPH methods, respectively. The radical ABTS*+ used in TEAC method is therefore the most reactive and responds to the highest number of hydroxyl groups of the phenolic compounds of wines.

Keywords: wine; phenolic compounds; total antioxidant activity; FCM; PBM; TEAC; DPPH; FR

Published: August 31, 2008  Show citation

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STRATIL P, KUBÁŇ V, FOJTOVÁ J. Comparison of the phenolic content and total antioxidant activity in wines as determined by spectrophotometric methods. Czech J. Food Sci. 2008;26(4):242-253. doi: 10.17221/1119-CJFS.
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    References

    1. Arnao M.B. (2000): Some methodological problems in the determination of antioxidant activity using chromogen radicals: a practical case. Trends in Food Science and Technology, 11: 419-421. Go to original source...
    2. Benzie I.F.F., Strain J.J. (1996): The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Analytical Biochemistry, 239: 70-76. Go to original source... Go to PubMed...
    3. Cooper K.A., Chopra M., Thurnham D.I. (2004): Wine polyphenols and promotion of cardiac health. Nutrition Research Review, 17: 111-129. Go to original source... Go to PubMed...
    4. Fernández-Pachón M.S., Villaňo D., Troncoso A.M., García-Parrilla M.C. (2006): Determination of phenolic composition of sherry and table white wines by liquid chromatography and their relation with antioxidant activity. Analytica Chimica Acta, 563: 101-108. Go to original source...
    5. Gardner P.T., White T.A.C., Mcphail D.B., Duthie G.G. (2000): The relative contribution of vitamin C, carotenoids and phenolics to the antioxidant potential of fruit juices. Food Chemistry, 68: 471-474. Go to original source...
    6. Gronbaek M., Deis A., Sorensen T.I.A., Becker U., Schnohr P., Jensen G. (1995): Mortality associated with moderate intakes of wine, beer, or spirits. British Medical Journal, 310: 1165-1169. Go to original source... Go to PubMed...
    7. Heinonen M., Lehtonen P.J., Hopia A.I. (1998): Antioxidant activity of berry and fruit wines and liquors. Journal of Agriculture and Food Chemistry, 46: 25-31. Go to original source... Go to PubMed...
    8. Hertog M.G.L., Feskens E.J.M., Hollman P.C H., Katan M.B., Kromhout D. (1993): Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen elderly study. Lancet, 342: 1007-1011. Go to original source... Go to PubMed...
    9. Hertog M.G.L., Kromhout D., Aravanis C., Blackburn H., Buzina R., Fidanza F., Giampaoli S., Jansen A., Menotti A., Nedeljkovic S., Pekkarinen M., Simic B.S., Toshima H., Feskens E.J.M., Hollman P.C.H., Katan M.B. (1995): Flavonoid intake and long-term risk of coronary heart disease and cancer in the seven countries study. Archives of Internal Medicine, 155: 381-386. Go to original source...
    10. Hoffmeister H., Schelp F.P., Mensink G.B.M., Dietz E., Bohning D. (1999): The relationship between alcohol consumption, health indicators and mortality in the German population. International Journal of Epidemiology, 28: 1066-1072. Go to original source... Go to PubMed...
    11. Huang D., Ou B.X., Prior, R.L. (2005): The chemistry behind antioxidant capacity assays. Journal of Agriculture and Food Chemistry, 53: 1841. Go to original source... Go to PubMed...
    12. Imeh U., Khokhar S. (2002): Distribution of conjugated and free phenols in fruits: antioxidant activity and cultivar variations. Journal of Agriculture and Food Chemistry, 50: 6301-6306. Go to original source... Go to PubMed...
    13. Jewell W.T., Ebeler S.E. (2001): Tyrosine biosensor for the measurement of wine polyphenolics. American Journal of Enology and Viticulture, 52: 219-222. Go to original source...
    14. Kim D.O., Lee K.W., Lee H.J., Lee C.Y. (2002): Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phytochemicals. Journal of Agriculture and Food Chemistry, 50: 3713-3717. Go to original source... Go to PubMed...
    15. Klatsky A.L., Armstrong M.A. (1993): Alcoholic beverage choice and risk of coronary artery disease mortality: do red wine drinkers fare best? American Journal of Cardiology, 71: 467-469. Go to original source... Go to PubMed...
    16. Knekt P., Järvinen R., Reunanen A., Maatela J. (1996): Flavonoid intake and coronary mortality in Finland: a cohort study. British Medical Journal, 312: 478-481. Go to original source... Go to PubMed...
    17. Lien E.J., Ren S., Bui H.H., Wang R. (1999): Quantitative structure-activity relationship analysis of phenolic antioxidants. Free Radical Biology & Medicine, 26: 285-294. Go to original source... Go to PubMed...
    18. Long L.H., Halliwell B. (2001): Antioxidant and prooxidant abilities of food and beverages. Methods in Enzymology, 335: 181-190. Go to original source... Go to PubMed...
    19. Lopes P., Drinkine J., Saucier C., Glories Y. (2006): Determination of l-ascorbic acid in wines by direct injection liquid chromatography using a polymeric column. Analytica Chimica Acta, 555: 242-245. Go to original source...
    20. Matějíček D., Mikeš O., Klejdus B., Štěrbová D., Kubáň V. (2005): Changes in content of phenolic compounds during maturing of barrique red wine. Food Chemistry, 90: 791-800. Go to original source...
    21. Miller N.J., Rice-Evans C.A., Davies M.J., Gopinathan V., Milner A. (1993): A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clinical Science, 84: 407-412. Go to original source... Go to PubMed...
    22. Ough C.S., Amerine M.A. (1988): Methods for Analysis of Musts and Wines. 2nd Ed. Wiley, New York.
    23. Pinto P.C.A.G., Saraiva M.L.M.F.S., Reis S., Lima J. L.F.C. (2005): Automatic sequential determination of the hydrogen peroxide scavenging activity and evaluation of the antioxidant potential by the 2,2´-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation assay in wines by sequential injection analysis. Analytica Chimica Acta, 531: 25-32. Go to original source...
    24. Plumb G.W., Price K.R., Williamson G. (1999): Antioxidant properties of flavonol glycosides from green beans. Redox Report, 4: 123-127. Go to original source... Go to PubMed...
    25. Price M.L., Butler L.G. (1977): Rapid visual estimation and spectrophotometric determination of tannin content of sorghum grain. Journal of Agriculture and Food Chemistry, 25: 1268-1273. Go to original source...
    26. Pulido R., Bravo L., Saura-Calixto F. (2000): Antioxidant activity of dietary polyphenols as determined by a modified FRAP assay. Journal of Agriculture and Food Chemistry, 48: 3396-3402. Go to original source... Go to PubMed...
    27. Re R., Pellegrini N., Proteggente A., Pannala A., Yang M., Rice-Evans C. (1999): Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology & Medicine, 26: 1231-1237. Go to original source... Go to PubMed...
    28. Renaud S.C., Gueguen R., Siest G., Salomon R. (1999): Wine, beer, and mortality in middle-aged men from eastern France. Archives of Internal Medicine, 159: 1865-1870. Go to original source... Go to PubMed...
    29. Renaud S., De Lorgeril M. (1992): Wine, alcohol, platelets and the French paradox for coronary heart disease. Lancet, 339: 1523-1526. Go to original source... Go to PubMed...
    30. Ribéreau-Gayon P., Dubourdieu D., Donéche B., Lonvaud A. (1998): Traité d'Oenologie - Microbiologie du Vin - Vinifications. Dunod, Paris: 282.
    31. Rice-Evans C.A., Miler N.J., Paganga G. (1996): Structure-antioxidant activity relationship of flavonoids and phenolic acids. Free Radical Biology & Medicine, 20: 933-956. Go to original source... Go to PubMed...
    32. Rimm E.B., Williams P., Fosher K., Criqui M., Stampfer M.J. (1999): Moderate alcohol intake and lower risk of coronary heart disease: meta-analysis of effects on lipids and haemostatic factors. British Medical Journal, 319: 1523-1528. Go to original source... Go to PubMed...
    33. Sánchez-Moreno C., Larrauri J.A., Saura-Calixto F. (1998): A procedure to measure the antiradical efficiency of polyphenols. Journal of the Science of Food and Agriculture, 76: 270-276. Go to original source...
    34. Sánchez-Moreno C., Larrauri J.A, Saura-Calixto F. (1999): Free radical scavenging capacity of selected red rosé and white wines. Journal of the Science of Food and Agriculture, 79: 1301-1304. Go to original source...
    35. Santos J.C.C., Korn M. (2006): Exploiting sulphide generation and gas diffusion separation in a flow system for indirect sulphite determination in wines and fruit juices. Microchimica Acta, 153: 87-94. Go to original source...
    36. Scalbert A., Manach C., Morand C., Rémésy C. (2005): Dietary polyphenols and the prevention of diseases. Critical Reviews in Food Science and Nutrition, 45: 1-20. Go to original source... Go to PubMed...
    37. Schoonen J.W., Sales M.G.F. (2002): Determination of polyphenols in wines by reaction with 4-aminoantipyrine and photometric flow-injection analysis. Analytical and Bioanalytical Chemistry, 372: 822-828. Go to original source... Go to PubMed...
    38. Simonetti P., Pietta P., Testolin G. (1997): Polyphenol content and total antioxidant potential of selected Italian wines. Journal of Agriculture and Food Chemistry, 45: 1152-1155. Go to original source...
    39. Singleton V.L. (1982): Grape and wine phenolics; background and prospects. In: Grape and Wine Centennial Symposium, Proceedings of the University of California, Davis: 215-227.
    40. Singleton V.L., Orthofer R., Lamuela-Raventós R.M. (1999): Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299: 152-178. Go to original source...
    41. Soleas G.J., Tomlinson G., Diamandis E.P., Goldberg D.M. (1997): Relative contribution of polyphenolic constituents to the antioxidant status of wines: Development of a predictive model. Journal of Agriculture and Food Chemistry, 45: 3995-4003. Go to original source...
    42. Stevanato R., Fabris S., Momo F. (2004): New enzymatic method for the determination of total phenolic content in tea and wine. Journal of Agriculture and Food Chemistry, 52: 6287-6293. Go to original source... Go to PubMed...
    43. Stratil P., Klejdus B., Kubáň V. (2006): Determination of total content of phenolic compounds and their antioxidant activity in vegetables - evaluation of spectrofotometric methods. Journal of Agriculture and Food Chemistry, 54: 607-616. Go to original source... Go to PubMed...
    44. Stratil P., Klejdus B., Kubáň V. (2007): Determination of phenolic compounds and their antioxidant activity in fruits and cereals. Talanta, 71: 1741-1751. Go to original source... Go to PubMed...
    45. Trichopoulou A., Lagiou P. (1997): Healthy traditional Mediterranean diet: an expression of culture, history, and lifestyle. Nutrition Review, 55: 383-389. Go to original source... Go to PubMed...
    46. Vinson J.A., Hao Y., Su X., Zubík L. (1998): Phenol antioxidant quantity and quality in foods: vegetables. Journal of Agriculture and Food Chemistry, 46: 3630-3634. Go to original source...
    47. Waterman P.G., Mole S. (1994): Extraction and chemical quantification. In: Waterman P.G., Mole S. (eds): Analysis of Phenolic Plant Metabolites. Methods in Ecology. Blackwell Publishing, Oxford: 66-103.
    48. Wildenradt H.L., Singleton V.L. (1974): The production of aldehydes as a result of oxidation of polyphenolic compounds and its relation to wine aging. American Journal of Enology and Viticulture, 25: 119-124. Go to original source...

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