Czech J. Food Sci., 2008, 26(1):58-64 | DOI: 10.17221/1135-CJFS

Fractionation and identification of some phenolics extracted from evening primrose seed meal

Lucia Zahradníková, ©tefan Schmidt, Zuzana Sékelyová, Stanislav Sekretár
Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovak Republic

Crude ethanol and ethyl acetate extracts of evening primrose (Oenothera biennis L.) seed meal were separated into fractions using Silicagel L 100/200 column chromatography and methanol as the mobile phase. Six fractions were obtained from the ethanol extract and five fractions from the ethyl acetate extract and their contents of total phenolics were determined. By means of HPLC, the active compounds were identified as catechin, gallic acid, caffeic acid, syringic acid, and ferulic acid.

Keywords: evening primrose; phenolic acids; HPLC; phenolic compounds

Published: February 29, 2008  Show citation

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Zahradníková L, Schmidt ©, Sékelyová Z, Sekretár S. Fractionation and identification of some phenolics extracted from evening primrose seed meal. Czech J. Food Sci. 2008;26(1):58-64. doi: 10.17221/1135-CJFS.
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    References

    1. Amarowicz R., Shahidi F. (1994): Application of Sephadex LH-20 chromatography for separation of cyanogenic glycosides and hydrophilic phenolic fraction from flaxseed. Journal of Liquid Chromatography, 17: 1291-1299. Go to original source...
    2. Amarowicz R., Zegarska Z., Pegg B.P., Karamać M., Kosińska A. (2007): Antioxidant and radical scavenging activities of a barley crude extract and its fractions. Czech Journal of Food Sciences, 25: 73-80. Go to original source...
    3. Daud A., Gallo A., Riera S. (2005): Antimicrobial properties of Phrygilanthus acutifolius. Journal of Ethnopharmacology, 99: 193-197. Go to original source... Go to PubMed...
    4. Hagerman A.E., Riedl K.M., Jones G.A., Sovik K.N. (1998): High molecular weight plant polyphenolics (tannins) as biological antioxidants. Journal of Agricultural and Food Chemistry, 46: 1887-1892. Go to original source... Go to PubMed...
    5. Hudson J.B.F. (1984): Evening primrose (Oenothera spp.) oil and seed. Journal of American Oil Chemists Society, 61: 540-543. Go to original source...
    6. Meyer A.S., Heinonen M., Frankel E.N. (1998): Antioxidant interactions of catechin, cyanidin, caffeic acid, quercetin and elagic acid on human LDL oxidation. Food Chemistry, 61: 71-75. Go to original source...
    7. Moure A., Cruz J.M., Franco D., Dominquez J.M., Sineiro J., Dominquez H., Nunez M.J., Parajo J.C. (2001): Natural antioxidants from residual sources. Food Chemistry, 72: 145-171. Go to original source...
    8. Naczk M., Shahidi F. (2004): Extraction and analysis of phenolics in food - review. Journal of Chromatography A, 1054: 95-111. Go to original source...
    9. Niklová I., Schmidt ©., Habalová K., Sekretár S. (2001): Effect of evening primrose extracts on oxidative stability of sunflower and rapeseed oils. European Journal of Lipid Science and Technology, 103: 299-306. Go to original source...
    10. Schmidt ©., Niklová I., Sekretár S. (1998): Antioxidant activity of the rapeseed and evening primrose meals. Bulletin of Food Research, 37: 257-265.
    11. Schmidt ©., Pokorný J., Vajdák M., Sekretár S., Gordon M.H. (2003a): Oilseeds as a source of antioxidants. Bulletin of Food Research, 42: 133-149.
    12. Schmidt ©., Niklová I., Pokorný J., Farkaą P., Sekretár S. (2003b): Antioxidant activity of evening primrose phenolics in sunflower and rapeseed oils. European Journal of Lipid Science and Technology, 105: 427-435. Go to original source...
    13. Schmidt ©., Pokorný J. (2005): Potential application of oilseeds as sources of antioxidants for food lipids - a review. Czech Journal of Food Sciences, 23: 93-102. Go to original source...
    14. Swain T., Hillis W.E. (1959): The phenolic constituents of Prunus domestica I. - The quantitative analysis of phenolic constituents. Journal of Science of Food and Agriculture, 10: 63-68. Go to original source...
    15. Wettasinghe M., Shahidi F. (2000): Scavenging of reactive species and DPPH free radicals by extracts of borage and evening primrose meals. Food Chemistry, 70: 17-26. Go to original source...
    16. Wettasinghe M., Shahidi F, Amarowicz R. (2002): Identification and quantification of low molecular weight phenolic antioxidants in seeds of evening primrose (Oenothera biennis L.). Journal of Agricultural and Food Chemistry, 50: 1267-1271. Go to original source... Go to PubMed...
    17. Xu L., Diosady L.L. (1997): Rapid method for total phenolic acid determination in rapeseed/canola meals. Food Research International, 30: 571-574. Go to original source...
    18. Zahradníková L., Schmidt ©., Sekretár S., Janáč L. (2007): Determination of the antioxidant activity of Ginkgo biloba leaves extract. Journal of Food and Nutrition Research, 46: 15-19.

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