Czech J. Food Sci., 2007, 25(4):182-188 | DOI: 10.17221/690-CJFS

Determination of polyphenols in beer by an effective method based on solid-phase extraction and high performance liquid chromatography with diode-array detection

Markéta Dvořáková, Petr Hulín, Marcel Karabín, Pavel Dostálek
Department of Fermentation Chemistry and Bioengineering, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic

The determination of polyphenols by spectrophotometric detection is complicated due to their low concentrations in beer. The beer samples have to be pre-concentrated before using the spectrophotometric detection for their quantification. An analytical method based on solid-phase extraction (SPE) and followed by high performance liquid chromatographic separation with diode-array detection is used for the determination of free gallic, protocatechuic, caffeic, p-coumaric, ferulic and salicylic acids, of (+)-catechin, (-)-epicatechin, and quercetin. These phenolic compounds participate in colloidal and sensory stability of beer. Six different SPE cartridges were tested and three different types of elution with the most appropriate solvents (acetonitrile, acetone and methanol) were used. The performance of the HPLC method was assessed by the evaluation of parameters such as absolute recovery, relative standard deviation (RSD - lower than 10%), the limit of quantification (LOQ), and the limit of detection (LOD). The polyphenol content in various types of Czech beer is presented.

Keywords: polyphenols; beer; HPLC; SPE; spectrophotometric detection

Published: August 31, 2007  Show citation

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Dvořáková M, Hulín P, Karabín M, Dostálek P. Determination of polyphenols in beer by an effective method based on solid-phase extraction and high performance liquid chromatography with diode-array detection. Czech J. Food Sci. 2007;25(4):182-188. doi: 10.17221/690-CJFS.
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    References

    1. Anonymous (1998): Analytica EBC (Analityca European Brewery Convention). 5th Ed. Verlag Hans Carl Getränke-Fachverlag, Nürnberg.
    2. Andersen M.L., Lauridsen R.K., Skibsted L.H. (2003): Optimizing the use of phenolic compounds in foods, Phytochemical Functional Foods. CRC Press LLC, Boca Raton: 315-346. Go to original source...
    3. Betes-Saura C., Andrés-Lacueva C., Lamuela-Raventos R.M. (1996): Phenolics in white free run juices and wines from Pened'es by High-Performance Liquid Chromatography. Journal of Agriculture and Food Chemistry, 44: 3040-3046. Go to original source...
    4. Čepička J., Karabín M. (2002): Polyfenolové látky piva - přirozené antioxidanty. Chemické Listy, 96: 90-95.
    5. Dvořáková M., Dostálek P. (2006): Analytical methods for determination of polyphenols in sweet wort, wort and beer. Kvasný Průmysl, 52: 111-114. Go to original source...
    6. Es-Safi N., Fulcrand H., Cheynier V., Moutonet M. (1999): Competition between (+)-catechin and (-)-epicatechin in acetaldehyde-induced polymerization of flavonols. Journal of Agriculture and Food Chemistry, 47: 2088-2095. Go to original source... Go to PubMed...
    7. Floridi S., Montanari L., Marconi O., Fantozzi P. (2003): Determination of free phenolic acids in wort and beer by coulometric array detection. Journal of Agriculture and Food Chemistry, 51: 1548-1554. Go to original source... Go to PubMed...
    8. García A.A., Grande B.C., Gádara J.S. (2004): Development of rapid method based on solid-phase extraction and liquid chromatography with ultraviolet absorbance detection for determination of polyphenols in alcohol-free beers. Journal of Chromatography, A, 1054: 175-180. Go to original source...
    9. Goupy P., Hugues M., Boivin P., Amiot M.J. (1999): Antioxidant compounds of barley (Hordeum vulgare) and malt extracts. Journal of the Science of Food and Agriculture, 79: 1625-1634. Go to original source...
    10. Guillen D.A., Merello F., Barroso C.G., PerezBustamante J.A. (1997): Solid phase extraction for sample preparation in the HPLC analysis of polyphenolic compounds in "Fino" sherry wine. Journal of Agriculture and Food Chemistry, 45: 403-406. Go to original source...
    11. Hayes J.P., Smyth M.R., McMurrough I. (1987): Comparison of electrochemical and ultraviolet detection in High-Performance Liquid Chromatography for the determination of phenolic compounds commonly found in beers. Analyst, 112: 1197-1207. Go to original source... Go to PubMed...
    12. Karagiannis S., Economou A., Lanaridis P. (2000): Phenolic and volatile composition of wines made from Vitis vinifera cv. Muscat lefko grapes from the island of Samos. Journal of Agriculture and Food Chemistry, 48: 5369-5375. Go to original source... Go to PubMed...
    13. Kenyhercz T.M., Kissinger P.T. (1977): A new approach to the phenolic components in beer. Application to the determination of sinapic, ferulic, and p-coumaric acids. Journal of Agriculture and Food Chemistry, 25: 959-961. Go to original source...
    14. McMurrough I., Roche G.P., Cleary K.G. (1984): Phenolic acid in beers and worts. Journal of the Institute of Brewing, 90: 181-187. Go to original source...
    15. Montanari L., Perreti G., Patella F., Giudi A., Fantozzi P. (1999): Organic and phenolic acids in beer. Lebensmittel-Wissenschaft & Technologie, 32: 535-539. Go to original source...
    16. Pascual-Teresa S., Santos-Buelga C., Rivas-Gonzalo J.C. (2000): Quantitative analysis of flavan-3-ols in spanish foodstuffs and beverages. Journal of Agriculture and Food Chemistry, 48: 5331-5337. Go to original source... Go to PubMed...
    17. Robbins R.J. (2003): Phenolic acids in foods: An overview of analytical methodology. Journal of Agriculture and Food Chemistry, 51: 2866-2887. Go to original source... Go to PubMed...
    18. Sánchez F.G., Carnero C., Heredia A. (1988): Determination of p-coumaric and ferulic acids in mixtures by isodifferential derivative spectrophotometry. Analytical Letters, 21: 1243-1257. Go to original source...
    19. Shahidi F., Naczk M. (1995): Food Phenolics; Sources, Chemistry, Effects, Applications. Technomic Publishing Co. Inc., Lancaster: 10-13.
    20. Skerikova V., Grynova L., Jandera P. (2004): Using CoulArray detector for analysis of natural antioxidants. Chemické Listy, 98: 343-348.
    21. Whittle N., Eldridge H. (1999): Identification of the polyphenols in barley and beer by HPLC/MS and HPLC/ electrochemical detection. Journal of the Institute of Brewing, 105: 89-99. Go to original source...

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