Czech J. Food Sci., 2006, 24(6):288-296 | DOI: 10.17221/3327-CJFS

Occurrence of Alternaria toxins in fibre flax, linseed, and peas grown in organic and conventional farms: Monitoring pilot study

Jana Králová1, Jana Hajšlová1, Jan Poustka1, Miroslav Hochman2, Marie Bjelková2, Lenka Odstrčilová2
1 Department of Food Chemistry and Analysis, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech Republic
2 AGRITEC, Research, Breeding and Service Ltd., Šumperk, Czech Republic

Fungi representing Alternaria spp. are ubiquitous pathogens that may under certain conditions cause spoilage of various food crops. Several Alternaria species are known producers of toxic secondary metabolites in some fruits and vegetables, nevertheless, only limited knowledge is available on the occurrence of these mycotoxins in legumes and/or oilseeds used for human nutrition. In the first part of the presented study, the analytical method employing reversed phase high-performance liquid chromatography (HPLC) coupled with fluorescence detection (FLD) was implemented to enable the examination of these food commodities for the presence of altenuene (AE), alternariol (AOH), and alternariol monomethyl ether (AME); the limits of detection were 1, 3 and 2 µg/kg for AE, AOH, and AME, respectively. Altogether 122 flax and 84 pea seed samples grown under organic and/or conventional farming conditions were analysed in the years 2002-2003. AME was detected in 20 flax seed samples; AE and AOH were present in only 2 and 4 samples, respectively. More frequent incidence of Alternaria toxins was recognised in fibre flax seeds as compared to linseed samples. Compared to the crops from the conventional farming, the concentrations of these mycotoxins found in positive organic samples were higher. No Alternaria mycotoxins were detected in the pea samples, probably due to the presence of antifungal compounds in the respective crop.

Keywords: Alternaria; fungi; altenuene; alternariol; alternariol monomethyl ether; mycotoxins; fibre flax seeds; linseed seeds; pea seeds

Published: December 31, 2006  Show citation

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Králová J, Hajšlová J, Poustka J, Hochman M, Bjelková M, Odstrčilová L. Occurrence of Alternaria toxins in fibre flax, linseed, and peas grown in organic and conventional farms: Monitoring pilot study. Czech J. Food Sci. 2006;24(6):288-296. doi: 10.17221/3327-CJFS.
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    References

    1. CABRAL K.M.S., ALMEIDA M.S., VALENTE A.P., ALMEIDA C.L., KURTENBACH E. (2003): Production of the active antifungal Pisum sativum defensin 1 (Psd1) in Pichia pastoris: overcoming the inefficiency of the STE13 protease. Protein Expression and Purification, 31: 115-122. Go to original source... Go to PubMed...
    2. CHELKOWSKI J., VISCONTI A. (ed.) (1992): Alternaria: Biology, Plant Diseases and Metabolites. Elsevier, Amsterdam.
    3. DELGADO T., GÓMEZ-CORDOVÉS C., SCOTT P.M. (1996): Determination of alternariol and alternariol monomethyl ether in apple juice using solid-phase extraction and high-performance liquid chromatography. Journal of Chromatography A, 731: 109-114. Go to original source... Go to PubMed...
    4. DELGADO T., GÓMEZ-CORDOVÉS C. (1998): Natural occurrence of alternariol and alternariol monomethyl ether in Spanish apple juice concentrates. Journal of Chromatography A, 815: 93-97. Go to original source... Go to PubMed...
    5. FENG-GIN L., YOSHIZAWA T. (2000): Alternaria mycotoxins in weathered wheat from China. Journal of Agricultural and Food Chemistry, 48: 2920-2924. Go to original source... Go to PubMed...
    6. FENTE C.A., JAIMEZ J., VÁZQUEZ B.I., FRANCO C.M., CEPEDA A. (1998): Determination of alternariol in tomato paste using solid phase extraction and highperformance liquid chromatography with fluorescence detection. Analyst, 123: 2277-2280. Go to original source... Go to PubMed...
    7. GIACOMELLI L., BOGGETTI H., AGNELLI H., ANUNZIATA J., SILBER J.J., CATTANA R. (1998): Relevant physicochemical factors in chromatographic separation of Alternaria alternata mycotoxins. Analytica Chimica Acta, 370: 79-89. Go to original source...
    8. LAU B.P.-Y., SCOTT P.M., LEWIS D.A., KANHERE S.R., CLÉROUX CH., ROSCOE V.A. (2003): Liquid chromatography-mass spectrometry and liquid chromatographytandem mass spectrometry of the Alternaria mycotoxins alternariol and alternariol monomethyl ether in fruit juices and beverages. Journal of Chromatography A, 998: 119-131. Go to original source... Go to PubMed...
    9. LEHMANN L., WAGNER J., METZLER M. (2005): Estrogenic and clastogenic potential of the mycotoxin alternariol in cultured mammalian cells. Food and Chemical Toxicology, 44: 398-408. Go to original source... Go to PubMed...
    10. LIU G.T., QIAN Y.Z., ZHANG P., DONG Z.M., SHI Z.Y., ZHEN Y.Z., MIAO J., XU Y.M. (1991): Relationship between Alternaria alternata and oesophageal cancer. IARC Scientific Publications, 105: 258-262.
    11. MOLINA P.G., ZÓN M.A., FERNÁNDEZ H. (2002): The electrochemical behaviour of altenuene mycotoxin and its acidic properties. Journal of Electroanalytical Chemistry, 520: 94-100. Go to original source...
    12. SCOTT P.M. (2001): Analysis of agricultural commodities and foods for Alternaria mycotoxins. Journal of Association of Official Analytical Chemists International, 84: 1809-1817. Go to original source...
    13. SCOTT P.M., WEBER D., KANHERE S.R. (1997): Gas-chromatography-mass spectrometry of Alternaria mycotoxins. Journal of Chromatography A, 765: 255-263. Go to original source... Go to PubMed...
    14. STINSON E.E., OSMAN S.F., HEISLER E.G., SICILIANO J., BILLS D.D. (1981): Mycotoxin production in whole tomatoes, apples, oranges, and lemons. Journal of Agricultural and Food Chemistry, 29: 790-792. Go to original source... Go to PubMed...
    15. TORRES A., GONZÁLEZ H.H., ETCHEVERRY M., RESNIK S.L., CHULZE S. (1998): Production of alternariol and alternariol monomethyl ether by isolates of Alternaria spp. from Argentinian maize. Food Additives & Contaminants, 15: 56-60. Go to original source... Go to PubMed...
    16. TOURNAS V.H., STACK M.E. (2001): Production of alternariol and alternariol monomethyl ether by Alternaria alternata grown on fruits at various temperatures. Journal of Food Protection, 64: 528-532. Go to original source... Go to PubMed...
    17. VISCONTI A., SIBILIA A. (1994): Alternaria toxins. In: MILLER J.D., THRENHOLM H.L. (eds): Mycotoxins in grain. Eagan Press, St. Paul: 315.
    18. WEIDENBORNER M. (ed.) (2001): Encyclopedia of Food Mycotoxins. Springer-Verlag, Berlin Heidelberg: 19-22.
    19. WOODY A.M., CHU F.S. (1992): Toxicology of Alternaria mycotoxins. In: CHELKOWSKI J., VISCONTI A. (eds): Alternaria: Biology, Plant Diseases and Metabolites. Elsevier, Amsterdam: 409.
    20. YE X.Y., NG T.B. (2003): Isolation of pisumin, a novel antifungal protein from legumes of the sugar snap pea Pisum sativum var. macrocarpon. Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology, 134: 235-240. http://ec.europa.eu/food/plant/protection/pesticides/legislation_en.htm Go to original source... Go to PubMed...

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