Czech J. Food Sci., 2006, 24(5):217-222 | DOI: 10.17221/3316-CJFS

The comparison of the methods for the identification of pathogenic serotypes and biotypes of Yersinia enterocolitica: Microbiological methods and PCR

Miroslava Vázlerová, Iva Steinhauserová

Department of Meat Hygiene and Technology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic

In this study, pathogenic strains of Y. enterocolitica were identified by microbiological and PCR methods. The samples were collected from pigs, cattle, poultry, and slaughter houses. Three common techniques were used to isolate Y. enterocolitica from the samples - ITC, PSB, and direct on the CIN. Primers A1/A2, Y1/Y2, and rfbC 1/rfbC 2 were used for the specific detection of the pathogenic strains of Y. enterocolitica. Traditional microbiological methods were found to be insufficient for the specific identification of the Y. enterocolitica pathogen. In comparison with PCR which was able to detect 149 strains, the biochemical test could detect only 138 species. These results show that the use of biochemical methods of cultivation did not allow the identification of all Y. enterocolitica pathogens. In total, 149 strains of pathogenic Y. enterocolitica were examined of which 120 were from pigs, 19 from poultry, 8 were cattle strains, and 2 came from the environments of slaughterhouses.

Keywords: Yersinia enterocolitica; pathogenic serotype; PCR detection; cultivation; DNA; identification; serotype; biotype

Published: October 31, 2006  Show citation