Czech J. Food Sci., 2006, 24(1):19-25 | DOI: 10.17221/3289-CJFS

Chemoprotective Effects of Broccoli Juice Treated with High Pressure

Lucie Mandelová, Jiří Totušek
Institute of Preventive Medicine, Faculty of Medicine, Masaryk University in Brno, Brno, Czech Republic

We investigated chemoprotective effects of broccoli juice, treated with high pressure method, using 500 MPa for a period of 10 minutes. By the use of this method, the conservation of nutritionally important substances occurs (for example vitamins, polyphenolic compounds, glucosinolates and other constituent substances). We followed the inhibition of mutagenicity of the model mutagen, N-nitroso-N-methylurea (MNU), by means of in vivo micronucleus test. After fourteen-day application of broccoli juice (0.2 ml/10 g of body weight of mouse) and after a single administration of MNU mutagen (50 mg/kg), a statistically significant decrease (p < 0.01) occurred of the number of micronuclei induced by the application of MNU. Broccoli juice treated with high pressure therefore seems to have a preventive potential against MNU-induced micronuclei formation in BALB/C mouse bone marrow cells.

Keywords: micronucleus test; broccoli; antimutagenicity; MNU

Published: February 28, 2006  Show citation

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Mandelová L, Totušek J. Chemoprotective Effects of Broccoli Juice Treated with High Pressure. Czech J. Food Sci. 2006;24(1):19-25. doi: 10.17221/3289-CJFS.
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    References

    1. AGRAWAL R.C., KUMAR S. (1998): Prevention of cyclophosphamide-induced micronucleus formation in mouse bone marrow by indol-3-carbinol. Food and Chemical Toxicology, 36: 975-977. Go to original source... Go to PubMed...
    2. BRESNICK E., BIRT D.F., WOLTERMAN K., WHEELER M., MARKIN R.S. (1990): Reduction in mammary tumorogenesis in the rat by cabbage and cabbage residue. Carcinogenesis, 11: 1159-1163. Go to original source... Go to PubMed...
    3. CONAWAY C.C., YANG Y., CHUNG F. (2002): Isothiocyanates as cancer chemopreventive agents: Their biological activities and metabolism in rodents and humans. Current Drug Metabolism, 3: 233-255. Go to original source... Go to PubMed...
    4. CONSTANTINOU A.I., MEHTA R.G., VAUGHAN A. (1996): Inhibition of N-methyl-N-nitrosourea-induced mammary tumors in rats by the soybean isoflavones. Anticancer Research, 16: 3293-3298.
    5. DONELLY E.T., BARDWELL H., THOMAS G.A., WILLIAMS E.D., HOPER M., CROWE P., MCCLUGGAGE W.G., STEVENSON M., PHILLIPS D.H., HEWER A., OSBORNE M.R., CAMPBELL F.C. (2004): Modulation of N-methyl-Nnitrosourea-induced crypt restricted metallothionein immunopositivity in mouse colon by a non-genotoxic diet-related chemical. Carcinogenesis, 25: 847-855. Go to original source... Go to PubMed...
    6. EDENHARDER R., FRANGART J., HAGER M., HOFMANN P., RAUSCHER R. (1998): Protective effects of fruits and vegetables against in vivo clastogenicity of cyclophosphamide or benzo[a]pyrene in mice. Food and Chemical Toxicology, 36: 637-645. Go to original source... Go to PubMed...
    7. EDENHARDER R., KRIEG H., KÖTTGEN V., PLATT K.L. (2003): Inhibition of clastogenicity of benzo[a]pyrene and of its trans-7,8-dihydrodiol in mice in vivo by fruits, vegetables, and flavonoids. Mutation Research, 537: 169-181. Go to original source... Go to PubMed...
    8. FAHEY J.W., ZHANG Y., TALALAY P. (1997): Broccoli sprouts: An exceptionally rich source of inducers of enzymes that protect against chemical carcinogens. Proceedings of the National Academy of Sciences of the United States of America, 94: 10367-10372. Go to original source... Go to PubMed...
    9. FIMOGNARI C., BERTI F., IORI R., CANTELLI-FORTI G., HRELIA P. (2005): Micronucleus formation and induction of apoptosis by different isothiocyanates and a mixture of isothiocyanates in human lymphocyte cultures. Mutation Research, 582: 1-10. Go to original source... Go to PubMed...
    10. HUGHES R., POLLOCK J.R., BINGHAM S. (2002): Effect of vegetables, tea, and soy on endogenous N-nitrosation, fecal ammonia, and fecal water genotoxicity during a high red meat diet in humans. Nutrition and Cancer, 42: 70-77. Go to original source... Go to PubMed...
    11. KASSIE F., UHL M., RABOT S., GRASL-KRAUPP B., VERKERK R., KUNDI M., CHABICOVSKY M., SCHULTE-HERMANN R., KNASMÜLLER S. (2003): Chemoprevention of 2-amino-3-methylimidazo[4,5-f] quinoline (IQ)-induced colonic and hepatic preneoplastic lesions in the F344 rat by cruciferous vegetables administered simultaneously with the carcinogen. Carcinogenesis, 24: 255-261. Go to original source... Go to PubMed...
    12. KIM D.J., SHIN D.H., AHN B., KANG J.S., NAM K.T., PARK CH.B., KIM CH.K., HONG J.T., KIM Y., YUN Y.W., JANG D.D., YANG K. (2003): Chemoprevention of colon cancer by Korean food plat components. Mutation Research, 523-524: 99-107. Go to original source... Go to PubMed...
    13. KRISHNA G., HAYASHI M. (2000): In vivo rodent micronucleus assay: protocol, conduct and data interpretation. Mutation Research, 455: 155-166. Go to original source... Go to PubMed...
    14. LUTHY J., CARDEN B., FRIEDERICH U., BACHMANN M. (1984): Goitrin-a nitrosatable constituent of plant foodstuffs. Experientia, 15: 452-453. Go to original source... Go to PubMed...
    15. MALÝ I. (2000): Brokolice - nutričně hodnotná zelenina. Výživa a potraviny, 55: 44-46. Go to original source...
    16. MURILLO G., MEHTA R.G. (2001): Cruciferous vegetables and cancer prevention. Nutrition and Cancer, 41: 17-28. Go to original source... Go to PubMed...
    17. PAOLINI M., PEROCCO P., CANISTRO D., VALGIMIGLI L., PEDULLI G.F., IORI R., CROCE C.D., CANTELLI-FORTI G., LEGATOR M.S., ABDEL-RAHMAN S.Z. (2004): Induction of cytochrome P450, generation of oxidative stress and in vitro cell-transforming and DNA-damaging activities by glucoraphanin, the bioprecursor of the chemopreventive agent sulforaphane found in broccoli. Carcinogenesis, 25: 61-67. Go to original source... Go to PubMed...
    18. PAPAPOLYCHRONIADIS C. (2004): Environmental and other risk factors for colorectal carcinogenesis. Techniques in Coloproctology, 8: 7-9. Go to original source... Go to PubMed...
    19. PARK E.J., PEZZUTO J.M. (2002): Botanicals in cancer chemoprevention. Cancer Metastasis Review, 21: 231-255. Go to original source... Go to PubMed...
    20. RAMBOUSKOVÁ J. (2002): Brokolice - nutriční význam a účinky podporující zdraví. Diabetologie, metabolismus, endokrinologie, výživa, 5: 38-42.
    21. RAUSCHER R., EDENHARDER R., PLATT K.L. (1998): In vitro antimutagenic and in vivo anticlastogenic effects of carotenoids and solvent extracts from fruits and vegetables rich in carotenoids. Mutation Research, 413: 129-142. Go to original source... Go to PubMed...
    22. SCHAFFER E.M., LIU J.Z., GREEN J., DANGLER C.A., MILNER J.A. (1996): Garlic and associated alyl sulfur components inhibit N-methyl-N-nitrosourea induced rat mammary carcinogenesis. Cancer Letters, 102: 199-204. Go to original source... Go to PubMed...
    23. SCHMID W. (1975): The micronucleus test. Mutation Research, 31: 9-15. Go to original source... Go to PubMed...
    24. SCHONHOF I., KRUMBEIN A., BRÜCKNER B. (2004): Genotypic effects on glucosinolates and sensory properties of broccoli and cauliflower. Nahrung, 48: 25-33. Go to original source... Go to PubMed...
    25. SEDMÍKOVÁ M., TUREK B., BÁRTA I., STROHALM J., ŠMERÁK P., HOUŠKA M., MÜLLEROVÁ J. (1999): Hodnocení antimutagenní aktivity tlakově ošetřené (paskalizované) a tepelně ošetřené (pasterované) květákové šťávy. Czech Journal of Food Sciences, 17: 149-152.
    26. SHAPIRO T.A., FAHEY J.W., WADE K.L., STEPHENSON K., TALALAY P. (2001): Chemoprotective glucosinolates and isothiocyanates of broccoli sprouts: Metabolism and excretion in humans. Cancer Epidemiology Biomarkers & Prevention, 10: 501-508.
    27. STEINKELLNER H., RABOT S., FREYWALD CH., NOBIS E., SCHARF G., CHABICOVSKY M., KNASMÜLLER S., KASSIE F. (2001): Effects of cruciferous vegetables and their constituents on drug metabolizing enzymes involved in the bioactivation of DNA-reactive dietary carcinogens. Mutation Research/Molecular Mechanisms of Anticarcinogenesis and Antimutagenesis, 480-481: 285-297. Go to original source... Go to PubMed...
    28. STEINMETZ K.A., POTTER J.D. (1996): Vegetables, fruit, and cancer prevention: a review. Journal of American Dietery Association, 96: 1027-1039. Go to original source... Go to PubMed...
    29. STEPHANOU G., VLASTOS D., VLACHODIMITROPOULOS D., DEMOPOULOS N.A. (1996): A comparative study on the effect of MNU on human cultures in vitro evaluated by O6-mdG formation, micronuclei and sister chromatid exchange induction. Cancer Letters, 109: 109-114. Go to original source... Go to PubMed...
    30. SUGIMURA T. (1986): Studies on environmental chemical carcinogenesis in Japan. Science, 233: 312-318. Go to original source... Go to PubMed...
    31. TALALAY P., FAHEY J.W. (2001): Phytochemicals from cruciferous plants protect against cancer by modulating carcinogen metabolism. Journal of Nutrition, 131: 3027-033. Go to original source... Go to PubMed...
    32. THORGEIRSSON U.P., DALGARD D.W., REEVES J., ADAMSON R.H. (1994): Tumor incidence in a chemical carcinogenesis study of nonhuman primates. Regulatory Toxicology and Pharmacology, 19: 130-151. Go to original source... Go to PubMed...
    33. TUREK B., HRUBÝ S., ČERNÁ M. (1994): Nutriční toxikologie. IDVPZ, Brno: 123.
    34. WEISBURGER J.H. (2000): Approaches for chronic disease prevention based on current understanding of underlying mechanisms. American Journal of Clinical Nutrition, 71: 1710-1714. Go to original source... Go to PubMed...

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