Czech J. Food Sci., 2005, 23(6):224-229 | DOI: 10.17221/3395-CJFS

Enzymatic activity in fermented milk products containing bifidobacteria

Iva Trojanová, Rada Vojtěch
Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Agriculture in Prague, Prague, Czech Republic

Streptococcus thermophilus, Lactobacillus delbrueckii subsp. bulgaricus and Bifidobacterium animalis strains were tested for á-galactosidase, â-galactosidase and á-glucosidase activities. Commercially available yoghurts with bifidobacteria were also tested. While bifidobacteria produced all the enzymes mentioned above, lactobacilli and streptococci exhibited only â-galactosidase activity. In yoghurts, only â-galactosidase was detected, while practically no á-galactosidase, and in one product only little á-glucosidase activities were exhibited. It could be concluded that the consumption of bifidobacteria via yoghurt has probably no substantial effect on the digestion of saccharides in the gut.

Keywords: fermented milk products; enzymatic activity; bifidobacteria

Published: December 31, 2005  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Trojanová I, Vojtěch R. Enzymatic activity in fermented milk products containing bifidobacteria. Czech J. Food Sci. 2005;23(6):224-229. doi: 10.17221/3395-CJFS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. CHARTERIS W., KELLY P., MORELLI L., COLLINS J. (1998): Ingredient selection criteria for probiotic microorganisms in functional dairy foods. International Dairy Technology, 51: 123-136. Go to original source...
    2. CHEVALIER P., ROY D., WARD P. (1990): Detection of Bifidobacterium species by enzymatic methods. Journal of Applied Bacteriology, 68: 619-624. Go to original source... Go to PubMed...
    3. CROSS M.L., STEVENSON L., GILL H.S. (2001): Antiallergy properties of fermented foods: an important immunoregulatory mechanism of lactic acid bacteria? International Immunopharmacology, 1: 891-901. Go to original source... Go to PubMed...
    4. FOOKS L.J., FULLER R., GIBSON G.R. (1999): Prebiotics, probiotics and human gut microbiology. International Dairy Journal, 9: 53-61. Go to original source...
    5. FULLER R. (1997): Introduction. In: FULLER R. (ed.): Probiotics 2. Applications and Practical Aspects. Chapman and Hall, London: 1-9. Go to original source...
    6. GIBSON G.R., ROBERFROID M.B. (1995): Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. Journal of Nutrition, 125: 1410-1412. Go to original source... Go to PubMed...
    7. GIBSON G.R., SAAVERDA J.M., MACFARLANE S., MACFARLANE G.T. (1997): Probiotics and intestinal infections. In: FULLER R. (ed.): Probiotics 2: Application and Practical Aspects. Chapman and Hall, London: 10-31. Go to original source...
    8. HASSAN A.N., FRANK J.F. (2001): Starter cultures and their use. In: MARTH E.H., STEELE J.L. (eds): Applied Dairy Microbiology. Marcel Dekker, Inc., New York: 151-206.
    9. HELLER K.J. (2001): Probiotics bacteria in fermented foods: product characteristics and starter organisms. American Journal of Clinical Nutrition, 73 (Supplement): 374S-379S. Go to original source... Go to PubMed...
    10. HELLER K.J. (2002): Will living starter bacteria no longer be an argument for the quality of fermented products? Nahrung, 46: 307. Go to original source... Go to PubMed...
    11. HUGHES B.B., HOOVER D.G. (1995): Viability and enzymatic activity of bifidobacteria in milk. Journal of Dairy Science, 78: 268-276. Go to original source... Go to PubMed...
    12. KOLARS J.C., LEVITT M.D., AOUJI M., SAVAIANO D.A. (1984): Yogurt - an autodigesting source of lactose. New England Journal of Medicine, 310: 1-3. Go to original source... Go to PubMed...
    13. LANGHENDRIES J.P., DETRY J., VAN HEES J., LAMBORAY J.M. (1995): Effect of a fermented infant formula containing viable bifidobacteria on the fecal flora composition and pH of healthy full-term infants. Journal of Pediatric Gastroenterology and Nutrition, 21: 177-181. Go to original source... Go to PubMed...
    14. MARSHALL V., COLE W.M. (1983): Threonine aldolase and alcohol dehydrogenase activities in Lactobacillus bulgaricus and Lactobacillus acidophilus and their contribution to flavour production in fermented milk. Journal of Dairy Research, 50: 375-379. Go to original source...
    15. MARSHALL V.M.E., TAMIME A.Y. (1997): Physiology and biochemistry of fermented milks. In: LOW B.A. (ed.): Microbiology and Biochemistry of Cheese and Fermented Milk. Elsevier Applied Science Publishers, London and New York: 153-192. Go to original source...
    16. MATSUKI T., WATANABE K., TANAKA R., FUKUDA M., OYAIZU H. (1999): Distribution of bifidobacterial species in human intestinal microflora examined with 16S rRNA-gene-targeted species-specific primers. Applied and Environmental Microbiology, 65: 4506-4512. Go to original source... Go to PubMed...
    17. MITSUOKA T. (1992): The human gastrointestinal tract. In: WOOD B.J.B. (ed.): The Lactic Acid Bacteria in Health and Disease. Elsevier Applied Science, London: 69-114. Go to original source...
    18. MODLER H.W., MCKELLAR R.C., YAGUCHI M. (1990): Bifidobacteria and bifidogenic factors. Canadian Institute of Food Science & Technology Journal, 23: 29-41. Go to original source...
    19. MOLIN G. (2001): Probiotics in foods not containing milk or milk constituents, with special reference to Lactobacillus plantarum 299v. American Journal of Clinical Nutrition, 73 (Supplement): 380S-385S. Go to original source... Go to PubMed...
    20. ORBAN J.I., PATTERSON, J.A (2000): Modification of the phosphoketolase assay for rapid identification of bifidobacteria. Journal of Microbiological Methods, 40, 221-224. Go to original source... Go to PubMed...
    21. PEREIRA D.I.A., GIBSON G.R. (2002): Cholesterol assimilation by lactic acid bacteria and bifidobacteria isolated from the human gut. Applied and Environmental Microbiology., 98: 4689-4693. Go to original source... Go to PubMed...
    22. RADA V. (1997): Detection of Bifidobacterium species by enzymatic methods and antimicrobial susceptibility testing. Biotechnology Techniques, 11: 909-912. Go to original source...
    23. RADA V., KOC J. (2000): The use of mupirocin for selective enumeration of bifidobacteria in fermented milk products. Milchwissenschaft, 55: 65-67.
    24. RASTALL R.A., GIBSON G.R. (2002): Prebiotic oligosaccharides: evaluation of biological activities and potential future developments. In: TANNOCK G.W. (ed.): Probiotics and Prebiotics Norfolk. Caister Academic Press, Norfolk: 107-148.
    25. REDDY B.S., RIVENSON A. (1993): Inhibitory effect of Bifidobacterium longum on collon, mammary and liver carcinogenesis induced by 2-amino-3-methylimidazo(4,5-f) quinoline, a food mutagen. Cancer Research, 53: 3914- 3918.
    26. ROSADO J.L., SOLOMONS N.W., ALLEN L.H. (1992): Lactose digestion from unmodified, low-fat and lactose-hydrolysed yogurt in adult lactose-maldigesters. European Journal of Clinical Nutrition, 46: 61-67.
    27. SAAVEDRA J.M., ABI-HANNA A., MOORE N., YOLKEN R. (1998): Effect of long term consumption of infant formulas with bifidobacteria and S. thermophilus on stool pattern and diaper rash in infants. Journal of Pediatric Gastroenterology and Nutrition, 27: 483-486. Go to original source...
    28. SCARDOVI V. (1986): Genus Bifidobacterium. In: SNEATH P.H.A., MAIR N.S., SHARPE M.E., HOLT J.G. (eds): Bergey's Manual of Systematic Bacteriology. Vol. 2. Williams and Wilkins, Baltimore: 1418-1434.
    29. TORRIANI S., VESCOVO M., DICKS L.M.T. (1997): Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus: a review. Annali di microbiologia ed enzimologia, 47: 29-52.
    30. VLKOVÁ E., MEDKOVÁ J., RADA V. (2002): Comparison of four methods for identification of bifidobacteria to the genus level. Czech Journal of Food Sciences, 20: 171-174. Go to original source...
    31. WANG R., CAO W.W., CERNIGLIA C.E. (1996): PCR detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples. Applied and Environmental Microbiology, 62: 1242-1247. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content