Czech J. Food Sci., 2005, 23(4):159-165 | DOI: 10.17221/3386-CJFS

the contents of trans fatty acids and cla in cow colostrum and milk fat in the early lactation period

Beata Paszczyk, Zofia Żegarska, Zbigniew Borejszo
Chair of Instrumental Analyses, Faculty of Food Sciences, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

The trans fatty acids as well as cis-9 trans-11 C18:2 (CLA) were determined in the colostrum and milk fat of individual cows during up to 82 days of lactation. The analyses were performed using gas chromatography (GLC) on a capillary column (CP Sil 88) combined with the method of argentation thin-layer chromatography (Ag-TLC). The results obtained in the study indicated that the milk fat of individual cows, kept under identical living and feeding conditions, was characterised by an extremely high variability of trans isomers concentration, especially of those of C18:1 acid. The differences referred not only to the total content of trans C18:1 isomers but also to the proportion of trans-10 and trans-11 isomers of C18:1. In some fat samples, a high concentration of trans C18:1 isomers was observed and trans-10 isomer of C18:1 was found to dominate. In the case of all the cows, the colostrum fat was characterised by a lower average content of the examined acids as compared to the milk fat. The average value of CLA in the colostrum fat of all the cows under study was 0.34%, and in the milk fat 0.42% of total fatty acids.

Keywords: milk fat; trans C18:1; trans C18:2; cis-9 trans-11 C18:2 (CLA); lactation period; GLC, TLC-AgNO3 analysis

Published: August 31, 2005  Show citation

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Paszczyk B, Żegarska Z, Borejszo Z. the contents of trans fatty acids and cla in cow colostrum and milk fat in the early lactation period. Czech J. Food Sci. 2005;23(4):159-165. doi: 10.17221/3386-CJFS.
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References

  1. ANONYM (1996): IDF standard: Milk: Determination of fat content - Gravimetric method 1D.
  2. ANONYM (1999): IDF standard: Milkfat: Preparation of fatty acid methyl esters 182.
  3. ANONYM (2002): PN-ISO 2446: Mleko. Oznaczanie zawartości tłuszczu.
  4. CHILLIARD Y., FERLAY A., MANSBRIDGE R.M., DOREAU M. (2000): Ruminant milk fat plasticity: Nutritional control of saturated, polyunsaturated, trans and conjugated fatty acids. Annales de zootechnie, 49: 181-205. Go to original source...
  5. GRIINARI J.M., DWYER D.A., MCGUIRE M.A., BAUMAN D.E., PALMQUIST D.L., NURMELA K.V.V. (1998): Transoctadecenoic acids and milk fat depression in lactating dairy cows. Journal of Dairy Science, 81: 1251-1261. Go to original source... Go to PubMed...
  6. HENNINGER M., ULBERTH F. (1994): Trans fatty acid content of bovine milk fat. Milchwissenschaft, 49: 555-558. JAWORSKI J., JAWORSKA H. (1973): Skład chemiczny tłuszczu mlekowego. VII. Ilościowe zmiany składu kwasów tłuszczowych tłuszczu mlekowego w okresie laktacji. Zeszyty Naukowe ART. Olsztyn Technologia Żywnosci, 1: 111-123.
  7. JIANG J., BJÖERCK L., FONDEN R., EMANUELSON M. (1996): Occurrence of conjugated cis-9,trans-11-octadecadienoic acid in bovine milk: Effects of feed and dietary regimen. Journal of Dairy Science, 79: 438-445. Go to original source... Go to PubMed...
  8. PALMQUIST D.L., BEAULIEU A.D., BARBANO D.M. (1993): Feed and animal factors influencing milk fat composition. Journal of Dairy Science, 76: 1753-1771. Go to original source... Go to PubMed...
  9. PARODI P.W. (1976): Distribution of isomeric octadecenoic fatty acids in milk fat. Journal of Dairy Science, 59: 1870-1873. Go to original source... Go to PubMed...
  10. PIPEROVA L.S., TETER B.B., BRUCKENTAL I., SAMPUGNA J., MILLS S.E., YURAWECZ M.P., FRITSCHE J., KU K., ERDMAN R.A. (2000): Mammary lipogenic enzyme activity, trans fatty acids and conjugated linoleic acids are altered in lactating dairy cows fed a milk fat-depressing diet. The Journal of Nutrition, 130: 2568-2574. Go to original source... Go to PubMed...
  11. PRECHT D., MOLKENTIN J. (1994): Trans-oktadecensäuren in Milchfette und Margarine. Kieler Milchwirtschaftliche Forschungsberichte, 46: 249-261.
  12. PRECHT D., MOLKENTIN J. (1996): Rapid analysis of the isomers of trans-octadecenoic acid in milk fat. International Dairy Journal, 6: 791-809. Go to original source...
  13. PRECHT D., MOLKENTIN J. (1997): Effect of feeding on trans positional isomers of octadecenoic acid in milk fats. Milchwissenschaft, 52: 564-568.
  14. PRECHT D., HAGEMEISTER H., KANITZ W., VOIGT J. (2002): Milk fat depression and the role of trans and CLA fatty acid isomers by feeding a high fiber diet with calcium soaps of fatty acids in early lactating dairy cows. Milchwissenschaft, 57: 518-522.
  15. WONSIL B.J., HERBEIN J.H., WATKINS B.A. (1994): Dietary and ruminally derived trans-18:1 fatty acids alter bovine milk lipids. The Journal of Nutrition, 124: 556-565. Go to PubMed...
  16. WOLFF R.L. (1994): Contribution of trans-18:1 acids from dairy fat to European diets. Journal of the American Oil Chemists' Society, 71: 277-283. Go to original source...
  17. ŻEGARSKA Z., PASZCZYK B., BOREJSZO Z. (1996): Trans fatty acids in milk fat. Polish Journal of Food and Nutrition Sciences, 5/46: 89-97.

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