Czech J. Food Sci., 2004, 22(10):S280-S282 | DOI: 10.17221/10681-CJFS

The determination of N-methylcarbamate pesticides using enzyme immunoassays with chemiluminescent detection

B. Mičková, P. Rauch, A. Montoya, E. Ferri, F. Fini, S. Girotti

¹ Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic

² Centro de Investigación e Innovación en Bioingenieria, Universidad Politécnica de Valencia, Valencia, Spain

³ Instituto di Scienze Chimiche, Universitá di Bologna, Bologna, Italy

In the present work, enzyme-linked immunosorbent assays (ELISAs) with chemiluminescent detection for the determination of carbofuran, carbaryl and methiocarb were developed and the analytical parameters of these assays were compared with those of ELISAs with colorimetric detection. The sensitivity of immunochemical methods was expressed as detection limit, linear working range, and I₅₀ value. In comparison with colorimetric ELISA, the ability of the chemiluminescent reagents to detect lower concentrations of HRP allowed to decrease the optimal antibody and conjugate concentrations and to reach better analytical parameters. The experimental comparison of the analytical performance of the ELISAs was carried out by analysing simply diluted fruit juices, spiked at different concentration levels with the above mentioned pesticides. Recovery values for both ELISAs were around 100% and no matrix effects were observed when fruit juices were diluted 1:20 or more.

Keywords: N-methylcarbamate pesticides; chemiluminescent and colorimetric ELISA; fruit juices

Published: December 31, 2004  Show citation