Czech J. Food Sci., 2004, 22(10):S133-S135 | DOI: 10.17221/10636-CJFS
Protein-lipid interactions during oxidation of liposomes
- Department of Applied Chemistry and Microbiology, Division of Food Chemistry, University of Helsinki, Helsinki, Finland, *E-mail: hanna.salminen@helsinki.fi
Oxidation of bovine serum albumin and its interaction with phenolic red raspberry and bilberry extracts (4.2 and 8.4 μg/ml) was investigated in a liposome system. Samples were incubated in the dark at 37°C with copper, and the extent of oxidation was measured by determing the loss of tryptophan fluorescence and the formation of protein carbonyls, conjugated diene hydroperoxides and hexanal. Both red raspberry and bilberry extracts inhibited lipid and protein oxidation. Red raspberry extract in 4.2 μg/ml concentration was the best inhibitor against both lipid and protein oxidation. In conclusion, oxidative deterioration due to protein-lipid oxidation is inhibited by phenolic compounds in berries.
Keywords: protein oxidation; liposome model; phenolic compounds; antioxidant
Published: December 31, 2004 Show citation
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