Czech J. Food Sci., 2003, 21(1):1-12 | DOI: 10.17221/3471-CJFS

13C CP/MAS NMR spectra of pectins: a peak-fitting analysis in the C-6 region

A. Synytsya, J. Opíková, J. Brus
Department of Carbohydrate Chemistry and Technology, Institute of Chemical Technology, Prague, Czech Republic; 2Institute of Macromolecular Chemistry, Czech Academy of Science, Prague, Czech Republic

13C CP/MAS NMR spectra of pectin samples were measured and interpreted. The influence of methylesterification and O-acetylation on chemical shifts of pectin carbons was studied using model pectate, pectinates, and their acetylated derivatives. The spectra were analysed also by peak fitting in C-6 region to obtain the values of methylation (DM), amidation (DAm) and acetylation (DAc), degrees. The results were in a good agreement with both convenient methods (photometry, elemental analysis) and the NMR method based on the relative areas of OCH3 (DM) and CH3 (DAc) resonances.

Keywords: 13C CP, MAS NMR; pectins; peak fitting; degrees of methylation (DM); degrees of acetylation (DAc)

Published: February 28, 2003  Show citation

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Synytsya A, Opíková J, Brus J. 13C CP/MAS NMR spectra of pectins: a peak-fitting analysis in the C-6 region. Czech J. Food Sci. 2003;21(1):1-12. doi: 10.17221/3471-CJFS.
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