Czech J. Food Sci., 2002, 20(3):95-97 | DOI: 10.17221/3517-CJFS

Immobilized metal ion chromatographic (IMAC) determination of ovomucoid in hen's egg white

A. Prošková, J. Kučera
Food Research Institute Prague, Prague, Czech Republic

An immobilized metal ion high-performance liquid chromatographic technique was developed for the determination of one of the egg allergens - ovomucoid - in egg white and food products containing egg white. The method was tested using standard samples as well as complete egg white and its reproducibility was determined. The possible application of this method is discussed. The method is based on the HPLC chromatography on the chelating column saturated with copper ions. The sample is applied to the column at pH 7.5 and eluted isocraticaly with a buffer containing 1.75 mM imidazole in the optimum case.

Keywords: ovomucoid; determination; HPL.C; egg white; analytical method; IMAC; HPLC

Published: June 30, 2002  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Prošková A, Kučera J. Immobilized metal ion chromatographic (IMAC) determination of ovomucoid in hen's egg white. Czech J. Food Sci. 2002;20(3):95-97. doi: 10.17221/3517-CJFS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. COKE S.K., SAMPSON H.A. (1997): Allergenic properties of ovomucoid in man. J. Immunol., 159: 2026-2032. Go to original source... Go to PubMed...
    2. KAMINOGAWA S., EOMOTO A., KURISAKI J., YAMAUCHI K. (1985): Monoclonal antibodies against hen's egg ovomucoid. J. Biochem. (Tokyo), 98: 1027-1032. Go to original source... Go to PubMed...
    3. TAKAHASHI K., HORIGUCHI M., BANDO N., TSUJI H., OGAWA T., ASAO T. (1999): Immunochemical characterization of ovomucoid from Japanese quail egg white using monoclonal antibodies. J. Nutr. Sci. Vitaminol. (Tokyo), 45: 491-500. Go to original source... Go to PubMed...
    4. URISU A., ANDO H., MORITA Y., WADA E., YASAKI T., YAMADA K., KOMADA K., T ORII S., GOTO M., WAKAMATSU T. (1997): Allergenic activity of heated and ovomucoid-depleted egg white. J. Allergy Clin. Immunol., 100: 171-176. Go to original source... Go to PubMed...
    5. ZHANG J.W., MINE Y. (1998): Characterization of IgE and IgG epitopes on ovomucoid using egg-white-allergic patients' sera. Biochem. Biophys. Res. Commun., 253: 124-127. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content