Czech J. Food Sci., 2002, 20(2):43-47 | DOI: 10.17221/3508-CJFS

Beta-galactosidase activity in psychrotrophic microorganisms and their potential use in food industry

P. Karasová, V. Spiwok, ©. Malá, B. Králová, N.J. Russell
2 and NICHOLAS J. RUSSELL
1 Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague,
2 Czech Republic; Department of Biological Chemistry, Imperial College University of London, U.K.

Twenty-one psychrotrophic resp. psychrophilic bacterial strains were screened for presence of b-galactosidase activity which showed 8 of them. b-Galactosidase activity of these strains was determined for 2 substrates - synthetic substrate (ONPG) and lactose - and also temperature profile of this enzyme was measured. b-Galactosidase from Arthrobacter sp. C2-2 not only proved the typical properties of cold-active enzyme, but it also preferred lactose as a substrate. Therefore, it was chosen for further isolation and purification and was found that it contains two b-galactosidase isoenzymes. One of them had strong preference for lactose and was able to catalyse transglycosylation reactions at low temperature. It has, thus, potential use in food technology.

Keywords: psychrotrophic microorganism; cold-active enzyme; b-galactosidase; lactose hydrolysis; transglycosylation

Published: April 30, 2002  Show citation

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Karasová P, Spiwok V, Malá ©, Králová B, Russell NJ. Beta-galactosidase activity in psychrotrophic microorganisms and their potential use in food industry. Czech J. Food Sci. 2002;20(2):43-47. doi: 10.17221/3508-CJFS.
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    References

    1. CRITTENDEN R.G., PLAYNE M.J. (1996): Production, properties and applications of food-grade oligosaccharides. Trends Food Sci. Technol., 7: 353-361. Go to original source...
    2. MAHONEY R.R. (1985): Modification of lactose and lactosecontaining dairy products with beta-galactosidase. In: FOX P.F. (ed.): Developments in Dairy Chemistry 3. Elsevier, London: 69-110. Go to original source...
    3. MODLER H.W. (1994): Bifidogenic factors - sources, metabolism and applications. Int. Dairy J., 4: 383-407. Go to original source...
    4. OKUYAMA H. et al. (1998): Cold-adapted microorganisms for use in food biotechnology. In: MARGESIN R., SCHINNER F. (eds.): Biotechnological Applications of Cold-Adapted Organisms. Springer, Berlin: 101-117. Go to original source...
    5. PAIGE D.A., DAVIS L.R. (1985): In: FOX P.F. (ed.): Developments in Dairy Chemistry 3. Elsevier, London: 111-133. Go to original source...
    6. RASTALL R.A., BUCKE C. (1992): Enzymatic synthesis of oligosaccharides. Biotechnol. Genet. Engng Rev., 10: 253- 281. Go to original source... Go to PubMed...
    7. SUAREZ F.L., SAVIANO D.A., LEVITT M.D. (1995): A comparison of symptoms after the consumption of milk or lactose-hydrolyzed milk by people with self-reported severe lactose intolerance. New England J. Med., 333:1-4. Go to original source... Go to PubMed...

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