Czech J. Food Sci., 2001, 19(6):207-212 | DOI: 10.17221/6609-CJFS
Verification of suitability of selected detection systems for estimating antibiotic residues in goat's milk
- Slovak University of Technology - Faculty of Chemical Technology, Bratislava, Slovak Republic
The objective of the paper was to verify the convenience of the application of three standardized detection systems: disk diffusion method, Delvotest SP and Penzym S 100 to control the antibiotic residues in goat's milk (β-lactam antibiotics and cephalosporins, aminoglycosides, tetracyclines, macrolides and others). It has been found that despite of certain specificity of goat's milk versus cow's milk the values of the majority of detection limits mutually correspond approximately to 90 %. The sensitivity of tests manifested itself in the following order: Penzym S 100 > Delvotest SP > disk diffusion method (the sensitivity was even several times lower). Inasmuch as the treatment of mastitis is carried out by using β-lactam antibiotics and cephalosporins, the above-indicated rapid methods (especially Penzym S 100 and Delvotest SP) can be recommended for the routine purposes of accomplishing a rapid hygienic control of goat's milk.
Keywords: goat's milk; disk diffusion method; Delvotest SP; Penzym S 100; antibiotics
Published: December 31, 2001 Show citation
References
- ECKSCHLAGER K., HORSÁK I., KODEJ Z. (1980): Vyhodnocování analytických výsledkù a metod. SNTL, Praha.
- H OFERICOVÁ M. (1999): Monosti stanovenia reziduí inhibièných látok. Mliekarstvo, 30: 2527.
Go to original source...
- H OZOVÁ B., GREIFOVÁ M., G ÖRNER F., ZEMANOVIÈ J. (1995): Porovnanie citlivosti diskovej difúznej metódy, Intestu a Delvotestu P pri stanovení reziduí antibiotík v mlieku. Potrav. Vìdy, 13: 131137.
Go to original source...
- H OZOVÁ B., HUDECOVÁ D., GREJTÁKOVÁ M. (1998): Hodnotenie Intestu, Delvotestu SP a Penzymu S 100 v porovnaní zo tandardizovanou diskovou difúznou metódou na detekciu β-laktámových antibiotík v mlieku. Czech J. Food Sci., 16: 6164.
- KLINGER I., ROSENTHAL I. (1997): Public health and the safety of milk and milk products from sheep and goats. Rev. Sci. Tech. Off. Int. Epiz., 16: 482488.
Go to original source...
Go to PubMed...
- MANN E.J. (1999): Goats milk. Dairy Ind. Int., 64: 1112.
- MARGETÍN M., MICHALÍK S. (1998): Sheep and goat husbandry in the Slovak Republic. FAO Reu Techn. Series, 50: 250-264.
- R YÁNEK D., SCHLEGELOVÁ M. (1993): Úèinnost BR-testu a Intestu pøi stanovení reziduí chemoterapeutik v syrovém a pasterizovaném mléce. Vet. Med. Czech, 38: 215222.
- SUHREN G., REICHMUTH J. (1998): Neue Tests-Neue Aspekte. β-Laktamantibiotikarückständen in Milch-Erfährungen mit dem SNAP-Beta-Laktamtest. DMZ, Lebensmittelindustrie und Milchwirtschaft, 119: 674681.
- SUHREN G., REICHMUTH J., WALTE H.G. (1996): Detection of β-lactam antibiotics in milk by the Penzym-test. Milchwissenschaft, 51: 269273.
- Z ENG S.S., ESCOBAR E.N., BROWN-GRONDER I. (1996): Evaluation of screening tests for detection of antibiotic residues in goat milk. Small Ruminant Res., 21: 155160.
Go to original source...
- ZENG S.S., HART S., ESCOBAR E.N., TESFAI K., GARZA E.K. DE (1998): Validation of antibiotic residue tests for dairy goats. J. Food Protect., 61: 344349.
Go to original source...
Go to PubMed...
- YAMANI I.M., AL-KURDI LINA M.A., HADDADIN M.Y., ROBINSON R.K. (1998): The detection of inhibitory substances in ex-farm milk supplies. Recent Res. Devel. Agric. Food Chem., 2: 611627.
- PENZYM S 100: Documentation HERSTELLER UCB BIOPRODUCTS, Belgium.
- Potravinový kódex Slovenskej republiky, roè. XXVIII, 1996, èiastka 14, èas F: 272272c..
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.