Czech J. Food Sci., 2001, 19(5):166-170 | DOI: 10.17221/6602-CJFS

Effect of yeast extract supplementation on β-galactosidase activity of Lactobacillus delbrueckii subsp. bulgaricus 11842 grown in whey.

D. Bury, J. Geciova, P. Jelen
2 1 DEAN BURY , JANA GECIOVA and PAVEL JELEN
1 Department of Agricultural, Food and Nutritional Science, Agricultural/Forestry Center,
2 University of Alberta, Edmonton, Canada; Institute of Chemical Technology - Department of Dairy and Fat Technology, Prague, Czech Republic

Lactobacillus delbrueckii subsp. bulgaricus 11842 (LB 11842) was grown in reconstituted sweet whey supplemented with 0 to 1% yeast extract. The β-galactosidase activity was determined from the hydrolysis of o-nitrophenyl- β-D-galactopyranoside (ONPG) by sonicated cultures. Shake flask cultures containing 0.2-0.8% yeast extract gave 2.5 times higher β-galactosidase activity than LB 11842 grown in sole (intact) whey. When supplementation with 1% yeast extract resulted even in a threefold increase of β-galactosidase activity. The rate of acid production increased with the addition of yeast extract, while the increase in the total cell count with increasing amount of yeast extract was slight. A maximum β-galactosidase activity of 1.08 ± 0.15 µmol ONP released per min per ml of culture was obtained using a whey based growth medium supplemented with 1% yeast extract.

Keywords: β-galactosidase; Lactobacillus delbrueckii subsp. bulgaricus 11842; yeast extract; whey

Published: October 31, 2001  Show citation

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Bury D, Geciova J, Jelen P. Effect of yeast extract supplementation on β-galactosidase activity of Lactobacillus delbrueckii subsp. bulgaricus 11842 grown in whey. Czech J. Food Sci. 2001;19(5):166-170. doi: 10.17221/6602-CJFS.
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