Czech J. Food Sci., 2001, 19(5):161-165 | DOI: 10.17221/6601-CJFS

Unusual behavior of natural polyphosphates during IMAC

M. Kmínková, A. Proąková, J. Kučera
Food Research Institute Prague, Prague, Czech Republic

The atypical course of IDA-ferric (Imino-Di-Acetyl-agarose saturated with ferric ions) of two polyphosphorylated compounds - phytic acid and Ser(P)-Ser(P)-Ser(P)-Glu-Glu cluster obtained by the way of pancreatic hydrolysis of bovine casein was detected. The spectral data (for phytic acid) and iron content (for polyphosphate cluster) indicate the strong bond of ferric ions to these polyphosphorylated compounds. The data shows these compounds to bind ferric ion stronger even in comparison with IDA (Imino-Di-Acetate) itself. Therefore attention is to be paid to all IMAC (Immobilized-Metal-Ion-Affinity-Chromatography) of natural compounds containing strong chelating groups or several chelating groups in vicinity.

Keywords: phytic acid; Ser(P)-Ser(P)-Ser(P)-Glu-Glu cluster; affinity chromatography; immobilized metal ion; IMAC; IDA-ferric; atypical adsorption

Published: October 31, 2001  Show citation

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Kmínková M, Proąková A, Kučera J. Unusual behavior of natural polyphosphates during IMAC. Czech J. Food Sci. 2001;19(5):161-165. doi: 10.17221/6601-CJFS.
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    References

    1. ADAMSON N.J., REYNOLDS E.C. (1995): Characterization of multiply phosphorylated peptides selectively precipitated from a pancreatic casein digest. J. Dairy Sci., 78: 2653– 2659. Go to original source... Go to PubMed...
    2. ANDERSSON L., PORATH J. (1986): Isolation of phosphoproteins by immobilized metal (Fe3+) affinity chromatography. Anal. Biochem., 154: 250–254. Go to original source... Go to PubMed...
    3. MUSZYNSKA, G., ANDERSSON L., PORATH J. (1986): Selective adsorption of phosphoproteins on gel-immobilized ferric chelate. Biochemistry, 25: 6850–6853. Go to original source... Go to PubMed...
    4. MUSZYNSKA, G., DOBROWOLSKA G., MEDIN A., EKMAN P., PORATH J.O. (1992): Model studies on iron(III) ion affinity chromatography. II. Interaction of immobilized iron(III) ions with phosphorylated amino acids, peptides and proteins. J. Chromatogr., 604: 19–28. Go to original source... Go to PubMed...

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