Czech J. Food Sci., 2001, 19(4):143-147 | DOI: 10.17221/6598-CJFS

The determination of ferulic acid in sugar beet pulp

P. Jankovská, J. Čopíková, A. Sinitsya
Institute of Chemical Technology - Department of Carbohydrates Chemistry and Technology, Prague, Czech Republic

The content of ferulic acid in sugar beet pulp was determined by reverse phase high-pressure liquid chromatography (HPLC) and UV/VIS-spectroscopy. The acid extracts of pectin carrying feruloyl groups were prepared for analysis. To release ferulic acid from pectin the hydrolysis in alkaline medium (pH = 12.5) was performed. Both non-hydrolysed and hydrolysed extracts were measured by UV/VIS-spectroscopy after pH adjustment to the value of 10. The absorbance maximum was observed at 372 nm (ester of ferulic acid) for non-hydrolysed extracts and at 345 nm (sodium ferulate) for hydrolysed extracts. The HPLC estimation of ferulic acid was made in hydrolysed extracts only. The content of ferulic acid in sugar beet pulp was in the range of 0.3-0.9% (m/m). The data obtained by application of the particular methods to one set of samples were statistically compared. The results of all methods were in good agreement with each other\

Keywords: ferulic acid; sugar beet pulp; HPLC; UV, VIS-spectroscopy

Published: August 31, 2001  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Jankovská P, Čopíková J, Sinitsya A. The determination of ferulic acid in sugar beet pulp. Czech J. Food Sci. 2001;19(4):143-147. doi: 10.17221/6598-CJFS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. A NONYMUS (1996): Dictionary of natural products G–L. Chemical database. Charman and Hall: 3083.
    2. C ROTTY B.A., ZNAIDEN A.P., JOHNSONSON A. (1998): Ferulic acid with dimethyl isosorbide in cosmetic compositions, patent CA section 62 (Essential Oils and Cosmetics). US 5824326.
    3. FRY S.C. (1982): Phenolic components of the primary cell wall. Biochem. J., 203: 493–504. Go to original source... Go to PubMed...
    4. IIYAMA K., LAM T.B.T., STONE B.A. (1994): Covalent crosslinks in the cell wall. Plant Physiol., 104: 315–320. Go to original source... Go to PubMed...
    5. IZYDORCZYK M., BILIADERIS C.G. (1995): Cereal arabinoxylans advances in structure and physiochemical properties. Carbohydr. Polym., 28: 33–48. Go to original source...
    6. MAGA J.A., NAIM M., ZEHAVI U., NAGY S., ROUSEFF R.L. (1992): In: CHI-TANG HO, CHANG Y. LEE, MOU-TANG HUANG (Eds): Phenolic Compounds in Food and their Effects on Health I. Am. Chem. Soc., Washington DC: 170– 191.
    7. PUSSAYANAWIN V., WETZEL D. (1987): High-performance liquid chromatographic determination of ferulic acid in wheat milling fractions as a measure of bran contamination. J. Chromatogr., 391: 243–255. Go to original source... Go to PubMed...
    8. RALPH J., QUIDEAU S., GRABBER J., HATFIELD D. (1994): Identification and synthesis of new ferulic acid dehydrodimers present in grass cell walls. J. Chem. Soc. Perkin Trans., 1: 3485–3494. Go to original source...
    9. SAULNIER L., CRÉPEAU M.J., LAHAYE M., THIBAULT J.F., GARCIA-CONESA M.T., KROON A.P., WILLIAMSON G. (1999): Isolation and structural determination of two 5,5'-diferuloyl oligosaccharides indicate that maize heteroxylans are covalently cross-linked by oxidatively coupled ferulates. Carbohydr. Res., 320: 82–92. Go to original source...
    10. SCHOENROCK U., UNTIEDT S., KUX U., INOUE K. (1997): Use of tocopheryl ferulates for lightening the skin browning caused by UV radiation. Patent CA section 62 (Essential Oils and Cosmetics). DE 19615575.
    11. SMITH M.M., HARTLEY D.R. (1983): Occurrence and nature of ferulic acid substitution of cell-wall polysaccharides in Graminaceous plants. Carbohydr. Res., 118: 65–80. Go to original source...
    12. VINKX C.J.A., DELCOUR J.A. (1996): Rye (Secale Cereale L.) arabinoxylans: A critical rewiew. J. Cereal Sci., 24: 1– 14. Go to original source...
    13. VORAGEN A.G.J., SCHOLS H.A., PILNIK W. (1986): Determination of the degree of methylation and acetylation of pectins by HPLC. Food Hydrocoloids, 1: 65–70. Go to original source...
    14. VORAGEN A.G.J., BERGMANS M.E.F., OOSTERVELD A., SCHOLS H.A., BELDMAN G. (1994): Carbohydrates as organic raw materials III. In: VAN BEKKUM H., RÖPER H., VORAGEN F. (Eds): Carbohydrate Research Foundation. The Hague, The Netherlands.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content