Czech J. Food Sci., 2001, 19(4):125-131 | DOI: 10.17221/6595-CJFS

Assay of antibiotic detection limits in cow's milk model samples and comparison of sensitivity of various detection systems (disk diffusion method, Delvotest SP and Penzym S 100).

B. Hozová, M. Kratmüllerová
Slovak University of Technology - Faculty of Chemical Technology, Bratislava, Slovak Republic

The work was aimed at estimating the detection limits of 21 antibiotics (beta-lactam antibiotics, cephalosporins, aminoglycosides, macrolides and others) by means of three types of detection systems (disk diffusion method with Bacillus stearothermophilus var. calidolactis C 953, Delvotest SP and Penzym S 100) and to verify their sensitivity in the evaluation of the admissible maximum residual limits (MRL). The high sensitivity and the good correlation of results have been achieved mainly by applying the rapid methods such as Delvotest SP and Penzym S 100 versus the less sensitive disk diffusion method. In the next stage of the work, detection limits of the mutual combinations of antibiotics in milk were estimated. In the model experiment, the synergic effect between ampicillin and oxacillin, cefuroxime and trimethoprim and between cephalosporins (combination of cefazolin with cefoperhazon) was observed.

Keywords: antibiotics; disk diffusion method; Delvotest SP; Penzym S 100; synergism

Published: August 31, 2001  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Hozová B, Kratmüllerová M. Assay of antibiotic detection limits in cow's milk model samples and comparison of sensitivity of various detection systems (disk diffusion method, Delvotest SP and Penzym S 100). Czech J. Food Sci. 2001;19(4):125-131. doi: 10.17221/6595-CJFS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. ABETE M.C., GENTA E., SQUADRONE S. (1997): Estimation of tetracycline in milk by HPLC with a diode array detector. Ind. Aliment., 36: 753–755.
    2. ALBRECHT U., HAMMER P., HEESCHEN W. (1996): Chicken antibody based ELISA for the detection of spiramycin in raw milk. Milchwissenschaft, 51: 209–212.
    3. ALBRECHT U., WALTE H.G., HAMMER P. (1998): Detection of erythromycin in raw milk by an antibody-capture immunoassay. Rieler Milchwirts. Fors.-Ber., 50: 163–170.
    4. CHARM S.E., ZOMER E. (1995): The evaluation and direction of rapid detection, identification of antimicrobial drugs. In: Proc. Residues of antimicrobial drugs and other inhibitors in milk. Kiel, Germany, 28–31 Aug.: 224–233.
    5. C RUZ A.D., B OTISTA G.C.M., M ODOLO J.R., G OTT SCHALK A.F., LOPES C.A.M. (1998): In vitro activity of danofloxacin and 7 other antimicrobials against strains of Staphylococcus aureus isolated from bovine mastitis. Arq. Bras. Med. Veter., Zoot., 50: 369–373.
    6. EL-BARADEI G.A.H. (1997): Sensitivity enzyme linked immunosorbent assay for detection of chloramphenicol residues in milk and some affecting factors. Alexandria J. Agric. Res., 42: 35–41.
    7. HASSAN ABBASI, HELLENÄS K.E. (1998): Modified determination of dihydrostreptomycin in kidney, muscle and milk by HPLC. Analyst, 123: 2725–2727. Go to original source... Go to PubMed...
    8. HOZOVÁ, B., GREIFOVÁ M., GÖRNER F., ZEMANOVIÈ J. (1995): Porovnanie citlivosti diskovej difúznej metódy, INTESTU a DELVOTESTU P pri stanovení rezíduí antibiotík v mlieku. Potrav. Vìdy, 13: 131–137. Go to original source...
    9. H OZOVÁ B., H UDECOVÁ D., GREJTÁKOVÁ M (1998): Hodnotenie INTESTU, DELVOTESTU SP a PENZYMU S 100 v porovnaní so štandardizovanou diskovou difúznou metódou na detekciu β-laktámových antibiotík. Czech J. Food Sci., 16 : 61–64.
    10. IBACH A., PETZ M. (1998): HPLC integrated solid-phase extraction with photochemical post-column derivatization for the determination of oxacillin, cloxacillin and dicloxacillin in raw milk. Lebensmittel Unters. Fors., 207: 170–173. Go to original source...
    11. KUBALEC P., BRANDŠTETEROVÁ E., BEDNÁRIKOVÁ A. (1997): Determination of oxacillin, cloxacillin and dicloxacilin in milk, meat and cheese samples using HPLC and precolumn derivatization. Z. Lebensmittel-Unters. Fors., 205: 85–88. Go to original source...
    12. MICHALSKI M.M., ROLA J. (1999): Evaluation of new β s.t.a.r. 25 and β s.t.a.r. 100 tests (UCB Belgium) in the detection of β-lactam antibiotics in milk. Zycie Weter., 74: 328–330.
    13. SCHLIEPHAKE A. (1998): A comparative study of a newly developed agar-diffusion test and the brilliant black reduction tests in conjunction with an ELISA reader to measure antibiotic residues in milk. Milchwissenschaft, 53: 88–90.
    14. SUHREN G., REICHMUTH J., WALTE H.G. (1996): Detection of β-lactam antibiotics in milk by the Penzym test. Milchwissenschaft, 51: 269–273.
    15. SUHREN G., REICHMUTH J. (1998): Neun Tests-Neue Aspekte. β-Laktamantibiotikarückständen in Milch-Erfährungen mit dem SNAP-Beta-Laktamtest. DMZ, Lebensmittelind. Milchwirts., 119: 674–681.
    16. SUHREN G., KANAPPSTEIN K. (1998): Detection of incurred dihydrostreptomycin residues in milk by liquid chromatography and preliminary confirmation methods. Analyst. 123: 2797–2801. Go to original source... Go to PubMed...
    17. TAGUCHI S., YOSHIDA S., FUKUSHIMA S., HORI S. (1997): Rapid determination of tetracycline antibiotics in milk by HPLC using an on-line clean-up system. J. Food Hyg. Soc. Jap., 38: 259–264. Go to original source...
    18. EEC (1992): Community procedure for the establishment of maximum residue limits of veterinary medicinal products in foodstuffs of animal origin. O. J. Eur. Com., L 73: 8–14.
    19. IDF (1991): Detection and confirmation of inhibitors in milk and milk products. Doc. IDF, 258: 99.
    20. STN 57 0531 (1995): Stanovenie rezíduí antibiotík a látok inhibujúcich rast mliekárenských kultúr v mlieku a mlieènych výrobkoch. Res. Dairy Inst., Žilina: 33.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content