RT Journal Article
SR Electronic
A1 Mastihuba, V.
A1 Leporisová, B.
T1 A new amperometric sucrose biosensor based on fructose dehydrogenase
JF Czech Journal of Food Sciences
YR 2000
VO 18
IS 4
SP 121
OP 123
DO 10.17221/8329-CJFS
UL https://cjfs.agriculturejournals.cz/artkey/cjf-200004-0001.php
AB A new, ferricyanide mediated amperometric biosensor for estimation of sucrose in biological fluids is proposed based on fructose dehydrogenase coimmobilised with baker's yeast as a source of invertase on the surface of glassy carbon electrode. Potential +420 mV was applied to the electrode against saturated calomel electrode. Maximum current was observed at pH 6, linearity of response ranged from 0.01mM to 0.29mM. Concentration of potassium ferricyanide 2mM was found as the best value.