PT Journal
AU Mastihuba, V
   Leporisova, B
TI A new amperometric sucrose biosensor based on fructose dehydrogenase
SO Czech Journal of Food Sciences
PY 2000
BP 121
EP 123
VL 18
IS 4
DI 10.17221/8329-CJFS
DE biosensor; sucrose; fructose dehydrogenase; baker's yeas
AB A new, ferricyanide mediated amperometric biosensor for estimation of sucrose in biological fluids is proposed based on fructose dehydrogenase coimmobilised with baker's yeast as a source of invertase on the surface of glassy carbon electrode. Potential +420 mV was applied to the electrode against saturated calomel electrode. Maximum current was observed at pH 6, linearity of response ranged from 0.01mM to 0.29mM. Concentration of potassium ferricyanide 2mM was found as the best value.
ER